云南高校图书馆联盟文献共享服务平台- LUCIFERASE

LUCIFERASE: 作品数：81被引量：93H指数：4; 导出分析报告; 相关领域：医药卫生生物学更多>>; 相关作者：夏海滨李学燕梁醒财阮光萍潘兴华更多>>; 相关机构：陕西师范大学南方医科大学四川大学吉林大学更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划国家科技支撑计划更多>>

self-assembling split Nano luciferase-based assay for investigating Pseudomonas syringae effector secretion: 《Stress Biology》2024年第1期594-599,共6页Pei Miao Jian-Min Zhou Wei Wang; supported by grants from the Natural Science Foundation of China(32370322);the Hainan Excellent Talent Team,and the State Key Laboratory of Plant Genomics.; Many Gram-negative pathogens employ the type III secretion system(T3SS)to deliver effector proteins into host cells,thereby modulating host cellular processes and suppressing host immunity to facilitate pathogenesis a...; 关键词：Pseudomonas syringae T3SS effectors SECRETION Nano luciferase

Enhancing tissue regeneration studies with luciferase-transgenic rats and bioluminescence imaging:A comprehensive review: 《Chinese Journal of Plastic and Reconstructive Surgery》2024年第4期214-218,共5页Poh-Ching Tan Eiji Kobayashi Qingfeng Li Shuangbai Zhou; supported by grants from the National Natural Science Foundation of China(grant no.82272287);the Shanghai Clinical Research Center of Plastic and Reconstructive Surgery supported by the Science and Technology Commission of Shanghai Municipality(grant no.22MC1940300);the Cross-Disciplinary Research Fund of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(grant no.JYJC202215);the“National Double First-Class”and“Shanghai Top-Level”high education initiative at Shanghai Jiao Tong University School of Medicine and Shanghai Key Research Center-Shanghai Research Center for Plastic Surgery(grant no.2023ZZ02023).; Bioluminescence imaging(BLI)in rodent models has revolutionized preclinical research in recent decades,enabling precise and noninvasive observation of cellular and molecular processes in vivo.Among various bioluminesc...; 关键词：Firefly rat Fat grafting LUCIFERASE Stem cells Skin expansion

利用Luciferase修饰的T细胞检测间充质干细胞免疫抑制功能方法的建立与评价: 《中国医药生物技术》2024年第5期458-460,488,共4页孙成峰段海英岳鹏升杨倩张埏菲孙慧燕; 河北省创新能力提升计划项目(22567663H);河北省自然科学基金(H2022103010)。; 间充质干细胞(mesenchymal stem cells,MSC)是一种具有自我更新和多向分化潜能的多能干细胞。广泛存在于骨髓、脐带、胎盘、脂肪等组织中,具有向炎症部位募集、多向分化、自我更新以及释放可溶性生物因子和分泌多种旁分泌因子的特点,这...; 关键词：炎症部位间充质干细胞免疫抑制功能多能干细胞生物因子多向分化潜能受损细胞炎症反应

Integration of HiBiT into enteroviruses:A universal tool for advancing enterovirus virology research被引量：1: 《Virologica Sinica》2024年第3期422-433,共12页Rui Yu Xiaohong Li Peng Zhang Minghao Xu Jitong Zhao Jingjing Yan Chenli Qiu Jiayi Shu Shuo Zhang Miaomiao Kang Xiaoyan Zhang Jianqing Xu Shuye Zhang; supported by the National Natural Science Foundation of China(grant nos.82002135 and 82172250).; The utilization of enteroviruses engineered with reporter genes serves as a valuable tool for advancing our understanding of enterovirus biology and its applications,enabling the development of effective therapeutic a...; 关键词：ENTEROVIRUS Infectious clones Reporter genes LUCIFERASE Drug screening

Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo: 《Virologica Sinica》2023年第4期549-558,共10页Shangrui Guo Meng Xun Tingting Fan Xinyu Li Haoyan Yao Xiaozhen Li Bo Wu Hang Yang Chaofeng Ma Hongliang Wang; supported by the National Natural Science Foundation of China (82272309).; Coxsackievirus belongs to the Picornaviridae family and is one of the major pathogens that cause hand,foot and mouth disease(HFMD)in infants and children with potential serious complications and even deaths.The pathog...; 关键词：Coxsackievirus LUCIFERASE High-throughput screening Mouse model

mgr-mir-9 implicates Meloidogyne graminicola infection in rice by targeting the effector MgPDI: 《Journal of Integrative Agriculture》2023年第5期1445-1454,共10页TIAN Zhong-ling ZHOU Jia-yan ZHENG Jing-wu HAN Shao-jie; financially supported by the National Natural Science Foundation of China (32001877)。; MicroRNAs (miRNAs),a class of small non-coding RNAs,are crucial endogenous gene regulators in a range of animals,including plant-parasitic nematodes.Meloidogyne graminicola is an obligate sedentary endoparasite of ric...; 关键词：microRNA function MELOIDOGYNE graminicola deep SEQUENCING Dual-Luciferase REPORTER Assay System proteindisulfideisomerase

Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes: 《Acta Biochimica et Biophysica Sinica》2022年第10期1507-1517,共11页Fangyang Shao Lei Ci Jiahao Shi Fei Fang Bowen Yan Xijun Liu Xiangyu Yao Mengjie Zhang Hua Yang Zhugang Wang Jian Fei; supported by the grants from the National Key R&D Program of China(No.2019YFA0905900);the Science and Technology Commission of Shanghai Municipality(No.16DZ2280800).; Monocyte chemoattractant protein-1(MCP-1)plays a crucial role in various inflammatory diseases.To reveal the impact of MCP-1 during diseases and to develop anti-inflammatory agents,we establish a transgenic mouse line...; 关键词：bioluminescence imaging LUCIFERASE MP-1 MOUSE PROMOTER

利用生物发光成像示踪大鼠颅骨缺损中移植的骨髓间充质干细胞被引量：1: 《中国组织工程研究》2022年第25期3968-3973,共6页曾裕威黄闯魏建国段东明王簕; 国家自然科学基金(82072409),项目负责人:王簕;广东省自然科学基金(2019A1515012020),项目负责人:王簕。; 背景:生物发光成像是一种新兴的活体成像技术,目前尚未有运用该技术对移植干细胞在颅骨缺损活体示踪的研究。目的:探究Luciferase慢病毒转染大鼠骨髓间充质干细胞的最佳转染复数,进一步研究表达荧光素酶骨髓间充质干细胞在体外的有效性...; 关键词：干细胞骨髓间充质干细胞 Luciferase慢病毒生物发光成像活体成像系统活体示踪大鼠颅骨缺损组织工程

A dual-luciferase reporter system for characterization of small RNA target genes in both mammalian and insect cells被引量：4: 《Insect Science》2022年第3期631-644,共14页Zhongyuan Deng Yuting Zhang Leyao Li Xingcheng Xie Jinyong Huang Min Zhang Xinzhi Ni Xianchun Li; This work was supported by the National Science Foundation of China(NSFC)-Henan Joint major grant(U2004206);the State Key Laboratory of Cotton Biology(CB2020A06).; MicroRNAs(miRNAs)are regulatory RNA molecules that bind to target messenger RNAs(mRNAs)and affect the stability or translational efficiency of the bound mRNAs.Single or dual-luciferase reporter systems have been succe...; 关键词：HELICOVERPA masc miRNA9a mRNA degradation PROMOTER psiCHECK-2-TK

Local Conformational Constraint of Firefly Luciferase Can Affect the Energy of Bioluminescence and Enzyme Stability被引量：1: 《CCS Chemistry》2022年第5期1695-1707,共13页Chao Zhang Xiaoguang Bai Shengxi Chen Larisa M.Dedkova Sidney M.Hecht; supported by research grants R01GM12367 and R35GM140819 from the National Institutes of General Medical Sciences,NIH.; Conformational dynamics contribute importantly to enzyme catalysis,such that targeted conformational constraint may affect catalysis.Firefly luciferases undergo extensive structural change during catalysis;key residue...; 关键词：genetic code reprogramming modified ribosomes conformational constraint luciferase bioluminescence and stability protein modeling

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