Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes  

作　　者：Fangyang Shao Lei Ci Jiahao Shi Fei Fang Bowen Yan Xijun Liu Xiangyu Yao Mengjie Zhang Hua Yang Zhugang Wang Jian Fei 

机构地区：[1]School of Life Sciences and Technology,Tongji University,Shanghai 200092,China [2]Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [3]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China [4]Shanghai Engineering Research Center for Model Organisms,SMOC,Shanghai 201203,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第10期1507-1517,共11页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Key R&D Program of China(No.2019YFA0905900);the Science and Technology Commission of Shanghai Municipality(No.16DZ2280800).

摘　　要：Monocyte chemoattractant protein-1(MCP-1)plays a crucial role in various inflammatory diseases.To reveal the impact of MCP-1 during diseases and to develop anti-inflammatory agents,we establish a transgenic mouse line.The firefly luciferase gene is incorporated into the mouse genome and driven by the endogenous MCP-1 promoter.A bioluminescence photographing system is applied to monitor luciferase levels in live mice during inflammation,including lipopolysaccharide-induced sepsis,concanavalin A-induced T cell-dependent liver injury,CCl_(4)-induced acute hepatitis,and liver fibrosis.The results demonstrate that the luciferase signal induced in inflammatory processes is correlated with endogenous MCP-1 expression in mice.Furthermore,the expressions of MCP-1 and the luciferase gene are dramatically inhibited by administration of the anti-inflammatory drug dexamethasone in a septicemia model.Our results suggest that the transgenic MCP-1-Luc mouse is a useful model to study MCP-1 expression in inflammation and disease and to evaluate the efficiency of anti-inflammatory drugs in vivo.

关 键 词：bioluminescence imaging LUCIFERASE MP-1 MOUSE PROMOTER 

分 类 号：Q6[生物学—生物物理学]