NK细胞对结直肠癌细胞增殖的影响  

Effect of NK cells on proliferation of colorectal cancer cells

作　　者：刘素兵 叶子瑜 梁艳芳 曾今诚 LIU Subing;YE Ziyu;LIANG Yanfang;ZENG Jincheng(Department of Clinical Laboratory,Dongguan Municipal Shipai Hospital,Dongguan,Guangdong 523330,China;Dongguan Municipal Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research,Guangdong Medical University,Dongguan,Guangdong 523808,China;Guangdong Xianhua Institute for Medical Research,Dongguan,Guangdong 523808,China;Dongguan Municipal Key Laboratory of Molecular Immunopathology/Department of Pathology,Binhaiwan Central Hospital,Dongguan,Guangdong 523905,China)

机构地区：[1]东莞市石排医院检验科,广东东莞523330 [2]广东医科大学东莞市医学活性分子开发与转化重点实验室,广东东莞523808 [3]广东省宪华医学研究院,广东东莞523808 [4]东莞市分子免疫病理研究重点实验室/东莞市滨海湾中心医院病理科,广东东莞523905

出　　处：《重庆医学》2025年第1期18-23,共6页Chongqing Medical Journal

基　　金：广东省自然科学基金项目(2024A1515012831);广东省基础与应用基础研究基金项目(2021B1515140066)。

摘　　要：目的研究自然杀伤(NK)细胞对4种结直肠癌(CRC)细胞株增殖的影响,探讨过继性NK细胞免疫疗法治疗CRC的可行性,为CRC诊疗提供实验依据。方法采用Ficoll密度梯度离心法分离外周血单个核细胞,在体外诱导活化为NK细胞并扩增,采用细胞计数试剂盒-8(CCK-8)法检测NK细胞对CRC细胞株RKO、HCT15、HCT116、LoVo增殖的影响,统计学分析比较NK细胞对CRC细胞株的抑制率。结果在不同效靶比下,NK细胞对同一种靶细胞的抑制率差异有统计学意义(P<0.05);在不同靶细胞数量(5×10^(3)个vs.1×10^(4)个)下,NK细胞对靶细胞RKO(效靶比0.4∶1)、HCT15(效靶比0.4∶1和0.2∶1)、HCT116(效靶比3.2∶1、1.6∶1、0.8∶1、0.4∶1和0.2∶1)及LoVo(效靶比1.6∶1、0.8∶1、0.4∶1、0.2∶1和0.1∶1)的抑制率差异有统计学意义(P<0.05),其余同种细胞各效靶比不同靶细胞数量组间抑制率比较差异均无统计学意义(P>0.05)。在不同靶细胞数量下,NK细胞对4种CRC细胞株最高抑制率的效靶比均为12.8∶1。结论过继性NK细胞免疫疗法对CRC的早期干预和治疗有重要意义,且12.8∶1可能是一个安全且疗效较好的效靶比。Objective To investigate the effect of NK cells on the proliferation of four kinds of colorectal cancer(CRC)lines,and to explore the feasibility of adoptive NK cell immunotherapy in the treatment of CRC so as to provide an experimental basis for the diagnosis and treatment of CRC.Methods Peripheral blood mononuclear cells were isolated by the Ficoll density gradient centrifuge method,which were in vitro induced to activate as the NK cells and amplified.The CCK-8 method was used to detect the effect of NK cells on the proliferation of CRC cell lines RKO,HCT15,HCT116 and LoVo.The inhibition rate of NK cells on CRC cell lines was statistically analyzed and compared.Results The inhibitory rate of NK cells against the same target cells was significantly different at different effect target ratios(P<0.05).Under different number of target cells(5×10^(3)vs.1×10^(4)),the inhibitory rate of NK cells against RKO(effect-target ratio 0.4∶1),HCT15(effect-target ratio 0.4∶1 and 0.2∶1),HCT116(effect-target ratio 3.2∶1,1.6∶1,0.8∶1,0.4∶1 and 0.2∶1)and LoVo(effect-target ratio 1.6∶1,0.8∶1,0.4∶1,0.2∶1 and 0.1∶1)were significantly different(P<0.05),while no statistical differences were found among other groups(P>0.05).The effect-target ratio corresponding to the maximum inhibitory rate of NK cells against four CRC cell lines was 12.8∶1 under different target cell numbers.Conclusion Adoptive NK cell immunotherapy has an important significance for the early intervention and treatment of CRC,moreover 12.8∶1 may be a safe and effective effect-target ratio.

关 键 词：NK细胞 结直肠癌细胞 效靶比 细胞增殖 过继性细胞免疫疗法 

分 类 号：R730.51[医药卫生—肿瘤]