幼鼠卵巢扭转复位后对性成熟期扭转侧卵巢组织结构及生殖功能的影响  

Effects of ovarian torsional reduction in young rats on ovary tissue structure and reproductive function on torsional side in adult stage

作　　者：张中严 马洪 李凯鑫 赵鹏 周光旭 刘斌 郎光友 ZHANG Zhongyan;MA Hong;LI Kaixin;ZHAO Peng;ZHOU Guangxu;LIU Bin;LANG Guangyou(Department of Pediatric Surgery,Affiliated Hospital of Zunyi Medical University,Zunyi,Guizhou 563000,China;Longgang District Maternity and Child Healthcare Hospital,Shenzhen,Guangdong 518172,China)

机构地区：[1]遵义医科大学附属医院小儿外科,贵州遵义563000 [2]深圳市龙岗区妇幼保健院,广东深圳518172

出　　处：《重庆医学》2025年第1期31-36,共6页Chongqing Medical Journal

基　　金：遵义市科技计划项目(遵市科合HZ字〔2021〕23号)。

摘　　要：目的探讨性成熟前期SD大鼠卵巢扭转后,对处于不同状态的扭转卵巢(未坏死卵巢、扭疑似坏死卵巢、扭转坏死卵巢),均行复位保留,待性成熟期对扭转侧卵巢生殖功能的影响。方法选取3周龄SD大鼠32只,随机分为4组、每组8只。采用类似建立睾丸扭转动物模型的Turner法,制作卵巢扭转动物模型,任选一组行假手术作为对照组(CG组),并分别建立扭转未坏死卵巢复位组(NNTOD组)、扭转疑似坏死卵巢复位组(SNTOD组)及扭转坏死卵巢复位组(NTOD组)。术后饲养至8周龄即性成熟期时,采用脊椎脱臼法处死实验动物,切取扭转侧卵巢组织,分别采用苏木精-伊红(HE)染色及透射电镜检查卵巢组织形态学及线粒体结构改变;原位DNA末端标记法(Tunel法)检测卵巢细胞凋亡;ELISA法检测心脏离心血中卵泡刺激素(FSH)、黄体生成素(LH)及抗米勒管激素(AMH)水平。结果CG组、NNTOD组卵巢结构清晰,卵巢各级卵泡均发育良好;电镜检查显示正常线粒体。SNTOD组卵巢部分结构排列紊乱,各级生长卵泡数目减少;电镜检查显示可见少许损伤的线粒体。NTOD组卵巢结构排列明显紊乱,各级生长卵泡数目明显减少;电镜检查显示多见肿胀明显且损伤严重的线粒体。与CG组比较,SNTOD组及NTOD组中凋亡率明显升高(P<0.05);CG组与NNTOD组间比较差异无统计学意义(P>0.05);与NTOD组间比较,SNTOD组的凋亡率降低(P<0.05)。与CG组比较,SNTOD组及NTOD组LH、FSH水平升高,AMH水平降低(P<0.05);SNTOD组的LH、FSH水平低于NTOD组,AMH水平高于NTOD组(P<0.05)。结论性成熟前期大鼠扭转疑似坏死卵巢复位后,性成熟期扭转侧卵巢生殖功能受到轻微损伤;扭转坏死卵巢复位后,生殖功能明显受损。Objective To conduct the reductive reservation for torsional ovary in different states(.unnecrotic ovary,suspected necrotic torsional ovary and torsional necrotic ovary)after the ovarian torsion in prematurity SD rat,and to investigate its effect on the ovarian reproductive function in maturity stage.Methods A total of 32 SD rats with 3 weeks old were selected and randomly divided into 4 groups,8 cases in each group.The animal model of ovarian torsion was made by using the Turner method,which was similar to the animal model of testicular torsion,and either group was selected to undergo the sham operation as the control group(CG group).The non-necrotic torsional ovary detorsion group(NNTOD group),suspected necrotic torsional ovary detorsion group(SNTOD group)and necrotic torsional ovary detorsion group(NTOD group)were established respectively.When the animals were fed to sexual maturity at 8 weeks of age,the experimental animals were sacrificed by vertebral dislocation method,and the ovary tissues on the torsional side were cut for HE staining and transmission electron microscopy to examine the changes of ovarian histomorphology and mitochondrial structure.Ovarian cell apoptosis was detected by Tunel assay.ELISA method was used to detect the follicle-stimulating hormone(FSH),luteinizing hormone(LH)and anti-mullerian duct hormone(AMH)levels in centrifugal blood.Results The ovarian structure in the CG group and NNTOD group was clear,the ovarian follicles at different levels were developed well;the electron microscopy showed normal mitochondria.The ovarian partial structure in the SNTOD group was disorganized,the number of follicles at all levels of growth was decreased;electron microscopic examination showed a little damage of mitochondria.In the NTOD group,the arrangement of ovarian structure was obviously disordered,the number of growing follicles at all levels was significantly reduced;the electron microscopy showed that most of the mitochondria were obviously swollen and severely damaged.Compared with the CG gro

关 键 词：卵巢扭转 抗米勒管激素 细胞凋亡 透射电镜 大鼠 

分 类 号：R361[医药卫生—病理学]