Ki-67、MCM2、p16在宫颈鳞状上皮内病变中表达及意义  

Analysis of the expression and significance of Ki-67、MCM2 and p16 in cervical intraepithelial lesions

作　　者：温俊秀 张可心 王琦 宋慧茹 倪璐 宋旭东 WEN Junxiu;ZHANG Kexin;WANG Qi;SONG Huiru;NI Lu;SONG Xudong(Department of Pathology,the Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China)

机构地区：[1]华北理工大学附属医院病理科,河北唐山063000

出　　处：《广州医药》2025年第1期82-88,共7页Guangzhou Medical Journal

摘　　要：目的 探讨Ki-67、微小染色体维持蛋白2(MCM2)、p16在宫颈鳞状上皮内病变中的表达及其临床意义。方法 采用免疫组化检测Ki-67、MCM2、p16在宫颈炎症组14例、低级别鳞状上皮内病变(LSIL)组47例、高级别鳞状上皮内病变(HSIL)组49例中的表达情况,以病理结果作为金标准,对结果进行统计分析。结果 HSIL组中Ki-67、MCM2、p16阳性率均高于炎症组和LSIL组(均P<0.017)。HSIL组中Ki-67、MCM2过表达率均显著高于炎症组和LSIL组(均P<0.017)。随着宫颈病变级别增加,Ki-67及MCM2阳性范围从基底层至表层逐渐扩大。MCM2及Ki-67在LSIL组中表达模式多为基底层的非过表达模式,HSIL组多为中层及以上的过表达模式。Spearman相关性分析显示,MCM2和Ki-67在宫颈鳞状上皮内病变中的表达强度之间呈正相关(r=0.801,P<0.05);p16与MCM2在宫颈鳞状上皮内病变中的表达呈正相关(r=0.559,P<0.05);p16与Ki-67在宫颈鳞状上皮内病变中的表达呈正相关(r=0.478,P<0.05)。结论 p16阳性提示宫颈高级别鳞状上皮内病变。MCM2与Ki-67在宫颈鳞状上皮内病变中的表达具有较高一致性,MCM2可作为宫颈鳞状上皮内病变新的增殖标志物。Objective To investigate the expression and significance of Ki-67,MCM2 and p16 in cervical intraepithelial lesions.Methods The expressions of Ki-67,MCM2 and p16 in cervicitis group(14 cases),low-grade squamous intraepithelial lesion(LSIL)group(47 cases)and high-grade squamous intraepithelial lesion(HSIL)group(49 cases)were detected by immunohistochemistry.The pathological results were used as the gold standard for statistical analysis.Results The positive rates of Ki-67,MCM2 and p16 in HSIL group were significantly higher than those in cervicitis group and LSIL group(P<0.017).The over-expression rates of Ki-67,MCM2 in HSIL group were significantly higher than those in cervicitis group and LSIL group(P<0.017).With the increase of cervical lesion grade,the positive range of Ki-67 and MCM2 gradually expanded from basal layer to surface layer.The expression patterns of MCM2 and Ki-67 in LSIL group were mostly non-overexpressed in the basal layers,while those in HSIL group were mostly overexpressed in the middle layer and above.Spearman correlation analysis showed that the expression intensity of MCM2 and Ki-67 in cervical squamous intraepithelial lesions was positively correlated(r=0.801,P<0.05).There was a positive correlation between the expression of p16 and MCM2 in cervical squamous intraepithelial lesions(r=0.559,P<0.05).There was a positive correlation between the expression of p16 and Ki-67 in cervical squamous intraepithelial lesions(r=0.478,P<0.05).Conclusions Positive p16 indicates high-grade squamous intraepithelial lesion.The expression of MCM2 and Ki-67 in cervical intraepithelial lesions is highly consistent.MCM2 can be used as a new proliferative marker for cervical intraepithelial lesions.

关 键 词：宫颈鳞状上皮内病变 KI-67 微小染色体维持蛋白2 P16 

分 类 号：R737.33[医药卫生—肿瘤]