真姬菇多糖结构鉴定及其抗肿瘤活性  

Structure Identification and Antitumor Activity of Hypsizygus marmoreus Polysaccharides

作　　者：李欣霖 朱链 刘可 尹相力 陈长青 黄宇 丁祥 LI Xinlin;ZHU Lian;LIU Ke;YIN Xiangli;CHEN Changqing;HUANG Yu;DING Xiang(Key Laboratory of Nanchong City of Ecological Environment Protection and Pollution Prevention in Jialing River Basin,College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,Sichuan,China;Key Laboratory of Southwest China Wildlife Resources Conservation(Ministry of Education),College of Life Science,China West Normal University,Nanchong 637009,Sichuan,China;Xichong Xinghe Biotechnology Co.,Ltd.,Nanchong 637260,Sichuan,China)

机构地区：[1]西华师范大学环境科学与工程学院,嘉陵江流域生态环境保护与污染防治南充市重点实验室,四川南充637009 [2]西华师范大学生命科学学院,西南野生动植物资源保护教育部重点实验室,四川南充637009 [3]西充星河生物科技有限公司,四川南充637260

出　　处：《食品研究与开发》2025年第3期45-54,共10页Food Research and Development

基　　金：四川省科技厅农业科技成果转化资金项目(2022NZZJ0003);四川省科技厅应用基础面上项目(2022NSFSC0107);四川省转移支付科技成果转移转化示范项目(22ZYZFSF0009、23ZHSF0082);达州市市校合作项目(2021-S05)。

摘　　要：为明确真姬菇多糖(Hypsizygus marmoreus polysaccharide,HM-P)的结构组成及体外抗肿瘤活性,采用热水浸提法分离提取HM-P,利用凝胶渗透色谱、傅里叶变换红外光谱和高效液相色谱等技术鉴定HM-P的组成成分,采用细胞活力检测试剂盒(cell counting kit-8,CCK-8)以及细胞划痕法检验HM-P的体外抗肿瘤活性。结果表明,HM-P的重均分子量(weight average molecular weight,MW)为25232 Da,由半乳糖、葡萄糖、木糖以72.406∶17.661∶9.933的含量比例组成。HM-P能够显著地抑制肺癌细胞(A549)、结肠癌细胞(CT26.WT)、成纤维细胞(L929)和胃癌细胞(MFC)增殖(P<0.05)、迁移(P<0.05),以及极显著地抑制腹水癌细胞(S180)增殖(P<0.01),其中HM-P对胃癌细胞(MFC)的直接抑制作用最强,当HM-P终浓度为20μg/mL时,抑制率达到27.9%,迁移率达到19.0%。HM-P通过介导巨噬细胞(RAW264.7)上清液杀死肺癌细胞(A549)、结肠癌细胞(CT26.WT)、成纤维细胞(L929)、胃癌细胞(MFC)及腹水癌细胞(S180),发现均有极显著的杀伤效果(P<0.01),且对肺癌细胞(A549)的杀伤效果最好,当HM-P终浓度为20μg/mL时,杀伤率高达42.6%。To determine the structure composition and in vitro anti-tumor activity of Hypsizygus marmoreus polysaccharide(HM-P),HM-P was isolated and extracted by hot water extraction method.The HM-P components were identified by gel permeation chromatography,Fourier transform infrared spectroscopy,and high performance liquid chromatography.The in vitro anti-tumor activity of HM-P was examined by cell counting kit-8(CCK-8)and cell scratch method.The result showed that HM-P,with a weight average molecular weight(MW)of 25232 Da,was composed of galactose,glucose,and xylose in the content ratio of 72.406∶17.661∶9.933.HM-P could significantly inhibit the proliferation(P<0.05)and migration(P<0.05)of lung cancer cells(A549),colon cancer cells(CT26.WT),fibroblasts(L929),and mouse forestomach carcinoma(MFC).It could also highly significantly inhibit the proliferation of ascites cancer cells(S180)(P<0.01).The direct inhibition of HM-P on mouse forestomach carcinoma(MFC)was the strongest.When the final concentration of HM-P was 20μg/mL,the inhibition rate reached 27.9%and the mobility rate 19.0%.HM-P mediated the supernatant of macrophages(RAW264.7)to kill lung cancer cells(A549),colon cancer cells(CT26.WT),fibroblasts(L929),mouse forestomach carcinoma(MFC)and ascites cancer cells(S180).It was found that the killing effect was extremely significant(P<0.01),especially on lung cancer cells(A549).The killing rate of lung cancer cells(A549)was 42.6%when the final concentration of HM-P was 20μg/mL.

关 键 词：真姬菇 ITS序列鉴定 多糖组成 体外 抗肿瘤活性 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]