模拟运输胁迫对牛蛙幼蛙生理机能的影响  

Effects of Simulated Transportation Stress on Physiological Functions of Juvenile Bullfrog(Aquarana catesbeiana)

作　　者：梁雪莹 郑晓婷[2,3] 陈秋羽 谢静怡 董宏标[2,4] 李勇 杨金龙 陈成勋[1] 张家松 LIANG Xueying;ZHENG Xiaoting;CHEN Qiuyu;XIE Jingyi;DONG Hongbiao;LI Yong;YANG Jinlong;CHEN Chengxun;ZHANG Jiasong(College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China;Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province,Sanya Tropical Fisheries Research Institute,Lingshui 572426,China;Zhuhai Modern Agriculture Development Centre,Zhuhai 519000,China)

机构地区：[1]天津农学院/水产学院,天津300384 [2]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [3]上海海洋大学水产与生命学院,上海201306 [4]三亚热带水产研究院海南省深远海渔业资源高效利用与加工重点实验室,海南陵水572426 [5]珠海市现代农业发展中心,广东珠海519000

出　　处：《广东海洋大学学报》2024年第6期118-126,共9页Journal of Guangdong Ocean University

基　　金：广东省重点领域研发计划项目(2021B0202030001);广东省现代农业产业技术体系创新团队建设项目(2022KJ150);广东省驻镇帮镇扶村科技特派员项目(KTP20210259);广州市农村科技特派员项目(SL2022E04J00301)。

摘　　要：【目的】为牛蛙(Aquarana catesbeiana)幼蛙健康运输以及运输胁迫后养殖恢复管理提供科学依据。【方法】采用体质量为(32.09±7.60)g、体长为(7.33±0.42)cm的牛蛙幼蛙,在4℃条件下模拟不同运输时长[0(对照组)、12和24 h],研究运输胁迫及胁迫后养殖7 d对幼蛙存活率、生化指标及肝肠组织结构的影响。【结果】不同运输时长及运输后7 d的养殖对幼蛙存活率无显著影响(P>0.05),存活率均为100%。模拟运输12 h后,幼蛙肠道脂肪酶活性和肝脏丙二醛含量显著高于对照组(P<0.05)。运输12 h和24 h后,幼蛙血清中尿素氮、葡萄糖及血氨浓度显著升高(P<0.05),而肝脏脂质积累减少;24 h运输后,幼蛙肌酐浓度显著降低(P<0.05);肝糖原含量减少。模拟运输后养殖7d,幼蛙肠道淀粉酶和脂肪酶活性显著高于运输24h组,肝脏总抗氧化能力和丙二醛浓度也显著升高(P<0.05)。与对照组相比,模拟运输后养殖7d,幼蛙肠道淀粉酶、肝肠总抗氧化能力、肝脏过氧化氢酶及超氧化物歧化酶活性、肠道丙二醛含量显著升高(P<0.01),肠道过氧化氢酶及超氧化物歧化酶活性显著降低(P<0.01),血清葡萄糖浓度显著降低(P<0.01),血氨浓度显著升高(P<0.05),尿素氮浓度显著升高(P<0.01)。同时,肠道黏膜杯状细胞数逐渐增多,肝脏中脂质沉积明显增加。【结论】在4℃下24h内,运输时长对牛蛙幼蛙存活率无影响,但延长运输时间会导致肝组织损伤。在此条件下,最佳运输时间建议不超过24 h。运输胁迫后养殖7d,幼蛙通过自身调节可将部分肝脏结构恢复与运输前一致,肠道恢复则需更长时间。【Objective】This study provides a scientific basis for the healthy transportation of bullfrog(Aquarana catesbeiana)juveniles and their aquaculture and recovery management following transportation-induced stress.【Method】Juvenile bullfrogs with an average body mass of(32.09±7.60)g and an average body length of(7.33±0.42)cm were studied.Transportation durations of 0,12,and 24 hours were simulated at 4°C to evaluate the effects of transportation stress and subsequent 7-day post-transport cultivation on survival rates,biochemical parameters,and liver and intestinal tissue structures.【Results】Transportation duration and post-transport cultivation did not significantly affect survival rates,which remained 100%across all groups.However,after 12 hours of transportation,intestinal lipase activity and liver malondialdehyde levels were significantly elevated compared to the control group(P<0.05).Following 12 and 24 hours of transport,serum levels of blood urea nitrogen,glucose,and plasma ammonia increased significantly(P<0.05),while liver lipid accumulation decreased.Additionally,creatinine levels significantly decreased after 24 hours of transportation(P<0.05),and liver glycogen content was reduced.After 7 days of cultivation,intestinal amylase,and lipase activities were significantly higher than in the 24-hour transport group.Total antioxidant capacity and liver MDA content also increased significantly(P<0.05).After 7 days of cultivation,the intestinal amylase activity,T-AOC of the liver and intestine,CAT and SOD activity in the liver,and malondialdehyde content in the intestine were significantly higher than those in the control group(P<0.01),whereas the CAT and SOD in the intestine was significantly lower than that in the control group(P<0.01).Additionally,the plasma glucose concentration significantly decreased(P<0.01),while the serum ammonia concentration significantly increased(P<0.01),and the blood urea nitrogen concentration significantly increased(P<0.01).The number of goblet cells in the intestinal m

关 键 词：牛蛙幼蛙 运输时间 存活率 血清生化指标 肝肠损伤 

分 类 号：S981.13[农业科学—捕捞与储运]