Dissecting the cell microenvironment of ovarian endometrioma through single-cell RNA sequencing  

作　　者：Jiangpeng Wu Siyu Xia Wenting Ye Yan Sun Jing Cai Fubing Yu Haiping Wen Xiuwei Yi Taikang Li Mingwei Chen Jiayun Chen Ge Song Chuanbin Yang Yali Song Jigang Wang 

机构地区：[1]Department of Reproductive Medicine,Dongguan Maternal and Child Health Care Hospital,Dongguan 523000,China [2]Department of Urology,and Shenzhen Clinical Research Centre for Geriatrics,Shenzhen People’s Hospital,Southern University of Science and Technology,Shenzhen 518020,China [3]State Key Laboratory for Quality Ensurance andSustainable Use of Dao-di Herbs,Artemisinin Research Center,andInstitute of Chinese Materia Medica,China Academyof Chinese Medical Sciences,Beijing 100700,China [4]State Key Laboratory of Antiviral Drugs,School of Pharmacy,Henan University,Kaifeng 475004,China [5]Reproductive Medicine Center,Foshan Women and Children Hospital,Foshan 528000,China

出　　处：《Science China(Life Sciences)》2025年第1期116-129,共14页中国科学(生命科学英文版)

基　　金：supported from Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2023D003,CI2021B014);the National Key Research and Development Program of China(2022YFC2303600,2020YFA0908000);the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-C-202002);the CACMS Innovation Fund(CI2023E002,CI2021A05101,CI2021A05104);the Science and Technology Foundation o f Shenzhen(JCYJ20210324115800001);the Science and Technology Foundation of Shenzhen(Shenzhen Clinical Medical Research Center for Geriatric Diseases);the Shenzhen Medical Research Fund(B2302051);the National Natural Science Foundation of China(82201786);Guangdong Basic and Applied Basic Research Foundation(2021A1515110646);Guangdong-Dongguan Joint Fund Regional Cultivation Project(2021B1515140033);Dongguan Science and Technology of Social Development Program(20211800904742,20221800905732,20221800904462).

摘　　要：Ovarian endometrioma(OE),also known as“chocolate cysts,”is a cystic mass that develops in the ovaries due to endometriosis and is a common gynecological condition characterized by the growth of endometrial tissue outside the uterus,leading to symptoms such as dysmenorrhea,pelvic pain,and infertility.However,the precise molecular and cellular mechanisms driving this pathophysiology remain largely unknown,posing challenges for diagnosis and treatment.Here,we employed integrated single-cell transcriptomic profiling of over 52,000 individual cells from endometrial tissues of OE patients and healthy donors and identified twelve major cell populations.We identified notable alterations in cell type-specific proportions and molecular signatures associated with OE.Notably,the activation of IGFBP5^(+) macrophages with pro-inflammatory properties,NK cell exhaustion,and aberrant proliferation of IQCG^(+) and KLF2^(+) epithelium are key features and may be the potential mechanisms underlying the pathogenesis of OE.Collectively,our data contribute to a better understanding of OE at the single cell level and may pave the way for the development of novel therapeutic strategies.

关 键 词：OVARY high-throughput sequencing TRANSCRIPTOMICS gene expression analysis ligand-receptor analysis 

分 类 号：R737.3[医药卫生—肿瘤]