hsa_circ_0001618通过miR-184/LARP1通路对烧伤后皮肤成纤维细胞增殖、迁移和凋亡的影响  

Effects of hsa_circ_0001618 on the proliferation,migration,and apoptosis of post-burn skin fibroblasts through the miR-184/LARP1 pathway

作　　者：徐思达[1] 虞耀华 徐沛 潘艳艳 乐欣[1] 邬薇薇[1] 范友芬[1] Xu Sida;Yu Yaohua;Xu Pei;Pan Yanyan;Le Xin;Wu Weiwei;Fan Youfen(Department of Burns,Ningbo No.2 Hospital,Ningbo 315010,China)

机构地区：[1]宁波市第二医院烧伤科,315010

出　　处：《中华损伤与修复杂志(电子版)》2025年第1期61-67,共7页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：浙江省卫生健康委员会医学卫生科技项目(2021KY1004);宁波市医学与健康学科领军项目(2022-F17);宁波市医疗卫生高端团队重大攻坚项目(2023030615)。

摘　　要：目的探讨hsa_circ_0001618通过miR-184/LARP1通路对烧伤后皮肤成纤维细胞增殖、迁移和凋亡的影响。方法烧伤皮肤组织样本来源于2020年5月宁波市第二医院烧伤科收治的17例Ⅱ度或Ⅲ度烧伤患者。通过定量实时聚合酶链反应(qRT-PCR)检测成纤维细胞和组织样本中hsa_circ_0001618、miR-184及LARP1的表达。分别采用CCK-8法、Transwell实验以及流式细胞术分析成纤维细胞的增殖、迁移及凋亡情况。通过双荧光素酶报告基因和Western blot检测观察基因表达调控。采用生物信息学方法分析hsa_circ_0001618/miR-184/LARP1轴在烧伤后皮肤成纤维细胞增殖、迁移和凋亡中的作用。结果与正常皮肤组织相比,烧伤皮肤组织中miR-184的表达下调(0.413±0.348比0.122±0.097,P=0.001)。受热后皮肤成纤维细胞中miR-184的表达水平低于未经热处理的对照成纤维细胞,且在48 h时下调最为显著(0.994±0.028比0.173±0.019,t=3.120,P<0.001)。miR-184的过表达抑制了成纤维细胞的增殖和迁移,并促进其凋亡(P<0.05)。miR-184与成纤维细胞中的LARP1呈负相关(r=-0.468,P=0.032)。生物信息学分析显示,hsa_circ_0001618通过miR-184调控热损伤后成纤维细胞的增殖、迁移和凋亡。结论hsa_circ_0001618通过miR-184/LARP1通路抑制烧伤后皮肤成纤维细胞的增殖和迁移并促进其凋亡,可能是促进烧伤创面愈合的潜在治疗靶点。Objective To investigate the effects of hsa_circ_0001618 on the proliferation,migration,and apoptosis of post-burn skin fibroblasts through the miR-184/LARP1 pathway.Methods Skin tissue samples were obtained from 17 patients with second or third-degree burn admitted to the Burns Department of Ningbo No.2 Hospital in May 2020.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of hsa_circ_0001618,miR-184,and LARP1 in fibroblasts and tissue samples.Proliferation,migration,and apoptosis of fibroblasts were analyzed using CCK-8 assay,Transwell experiments,and flow cytometry,respectively.Gene expression regulation was observed through dual-luciferase reporter gene assays and Western blot.Bioinformatics methods were used to analyze the role of hsa_circ_0001618/miR-184/LARP1 axis in the proliferation,migration,and apoptosis of post-burn skin fibroblasts.Results Compared to normal tissues,the expression of miR-184 was downregulated in burn tissues(0.413±0.348 vs 0.122±0.097,P=0.001).The expression level of miR-184 in skin fibroblasts decreased after heat treatment compared to untreated control fibroblasts,with the most significant decrease at 48 h(0.994±0.028 vs 0.173±0.019,t=3.120,P<0.001).Overexpression of miR-184 inhibited the proliferation and migration,and promoted the apoptosis of fibroblasts(P<0.05).miR-184 was negatively correlated with LARP1 in fibroblasts(r=-0.468,P=0.032).Bioinformatics analysis showed that hsa_circ_0001618 regulated the proliferation,migration,and apoptosis in skin fibroblasts after thermal injury through miR-184.Conclusion hsa_circ_0001618 inhibits the proliferation and migration,and promotes the apoptosis of post-burn skin fibroblasts via the miR-184/LARP1 pathway,potentially serving as a therapeutic target to promote burn wound healing.

关 键 词：环状RNA 烧伤 成纤维细胞 hsa_circ_0001618 miR-184 

分 类 号：R73[医药卫生—肿瘤]