The transcriptome of MHV-infected RAW264.7 cells offers an alternative model for macrophage innate immunity research  

作　　者：Yun Liu Ting-Ting Feng Wei Tong Zhi Guo Xia Li Qi Kong Zhi-Guang Xiang 

机构地区：[1]Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS)and Comparative Medicine Center,Peking Union Medical College(PUMC),Beijing,P.R.China

出　　处：《Animal Models and Experimental Medicine》2025年第1期57-66,共10页动物模型与实验医学(英文)

基　　金：CAMs innovation Fund for Medical Sciences,Grant/Award Number:2022-12M-CoV19-005;National Key Projects,Grant/Award Number:2023YFF0724900 and 2021YFF0702802。

摘　　要：Background:Macrophages are the primary innate immune cells encountered by the invading coronaviruses,and their abilities to initiate inflammatory reactions,to main-tain the immunity homeostasis by differential polarization,to train the innate immune system by epigenic modification have been reported in laboratory animal research.Methods:In the current in vitro research,murine macrophage RAW 264.7 cell were infected by mouse hepatitis virus,a coronavirus existed in mouse.At 3-,6-,12-,24-,and 48-h post infection(hpi.),the attached cells were washed with PBS and harvested in Trizol reagent.Then The harvest is subjected to transcriptome sequencing.Results:The transcriptome analysis showed the immediate(3 hpi.)up regulation of DEGs related to inflammation,like Il1b and Il6.DEGs related to M2 differential po-larization,like Irf4 showed up regulation at 24 hpi.,the late term after viral infection.In addition,DEGs related to metabolism and histone modification,like Ezh2 were de-tected,which might correlate with the trained immunity of macrophages.Conclusions:The current in vitro viral infection study showed the key innated im-munity character of macrophages,which suggested the replacement value of viral infection cells model,to reduce the animal usage in preclinical research.

关 键 词：CORONAVIRUS innate immunity MACROPHAGE TRANSCRIPTOME 

分 类 号：R392[医药卫生—免疫学]