Evaluation of a user-friendly CSDS cage apparatus for studying depressive-like behaviors in rodents  

作　　者：Hao Zhang Dongmei Gao Minghu Hu Wanqing Zhou Muxuan Han Ya Sun Yang Zhang Jieqiong Wang Mingzhou Gao 

机构地区：[1]High-Level Key Disciplines of Traditional Chinese Medicine:Basic Theory of Traditional Chinese Medicine,National Administration of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan,China [2]Key Laboratory of Traditional Chinese Medicine Classical Theory,Ministry of Education,Shandong University of Traditional Chinese Medicine,Jinan,China [3]Experimental Center,Shandong University of Traditional Chinese Medicine,Jinan,Shandong,China [4]College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan,Shandong,China [5]Innovative Institute of Chinese Medicine and Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan,Shandong,China [6]Social Cooperation and Achievement Transformation Department,Shandong University of Traditional Chinese Medicine,Jinan,Shandong,China

出　　处：《Animal Models and Experimental Medicine》2025年第1期179-186,共8页动物模型与实验医学(英文)

基　　金：National Natural Science Foundation of China,Grant/Award Number:82204958 and 82305065;Natural Science Foundation of Shandong Province,Grant/Award Number:ZR2020ZD17;Traditional Chinese Medicine Emotional Disease and Brain Steady State Regulation Innovation Team,Grant/Award Number:2023KJ191;Medical and Health Science and Technology Development Plan Project of Shandong Province,Grant/Award Number:202105010467;Traditional Chinese Medicine Science and Technology Project of Shandong Province,Grant/Award Number:Q-2022059。

摘　　要：Background:Previously,a chronic social defeat stress(CSDS)model has been widely-adopted for assessing depressive-like behaviors in animals.However,there is still room for improvement in the CSDS model to safeguard study accuracy and the wel-fare of lab rodents.Our study team developed a novel,standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability.Methods:An innovative CSDS cage apparatus and water bottle was designed.To evaluate the effectiveness of the newly developed tools,a variety of animal models,including the tail suspension test(TST),sucrose preference test,forced swimming test(FST),novelty-suppressed feeding test,female urine sniffing test,and open field test(OFT),were adopted to assess depressive-like behaviors in mice.Fluoxetine treat-ment was also administered to observe the reversal effect,as part of the validation.Results:The CSDS cage apparatus resulted in the manifestation of depressive-like behaviors in the model mice.Significant reductions in sucrose preference and urine sniffing time were observed,while the OFT revealed decreased central zone total distance,residence time,and frequency of entry.Moreover,increased immobility was found in the FST and TST.Fluoxetine treatment was found to successfully reverse the modeling effect.Conclusion:The CSDS cage apparatus was validated for enhanced usability and ad-dressed the previous challenges of water bottle leakage and lab rodent welfare is-sues.The consistent results from multiple behavioral tests also supported real-world application of the apparatus,offering researchers a promising alternative to conven-tional rodent cages.

关 键 词：CSDS DEPRESSION experimental cage leak-proof water bottle methodology 

分 类 号：R68[医药卫生—骨科学]