电感耦合等离子体质谱法快速测定人血清中40余种矿物质元素的方法研究  

Study on Rapid Determination of More than 40 Mineral Elements in Human Serum by Inductively Coupled Plasma Mass Spectrometry

作　　者：姚雪漫 夏瑜[1,2] 张秋萍 蒋建荣[1,2] 王欣 柳旭艳[3] YAO Xue-man;XIA Yu;ZHANG Qiu-ping;JIANG Jian-rong;WANG Xin;LIU Xu-yan(Suzhou Center for Disease Control and Prevention,Suzhou 215100,China;Jiangsu Key Laboratory of Food Safety Risk Monitoring(Illegal Addition Field),Suzhou 215100,China;School of Public Health,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]苏州市疾病预防控制中心,江苏苏州215100 [2]江苏省食品安全风险监测重点实验室(非法添加领域),江苏苏州215100 [3]南京医科大学公共卫生学院,江苏南京211166

出　　处：《分析测试学报》2025年第2期356-363,共8页Journal of Instrumental Analysis

基　　金：江苏省苏州市科技计划项目(SKY2022210);苏州市重点学科卫生检验学项目(SZXK202117);江苏省食品安全风险检测重点实验室(非法添加领域)资助。

摘　　要：该文建立了人血清中40余种矿物质元素的快速测定方法。血清样品经含0.01%曲拉通Triton X-100、0.1%硝酸的稀释液直接稀释后,使用电感耦合等离子体质谱仪(ICP-MS)的动能歧视型(KED)碰撞模式进行测定。分别用内标法和外标法进行方法学考察,在选择的仪器工作条件下,大部分待测矿物质元素均获得了良好的线性关系(相关系数r>0.999),检出限为0.000 200~18.6μg/L。准确度测定结果显示,血清标准品L-1、L-2给出的15种矿物质元素的内标法和外标法检测结果均在认定范围内;对没有给出认定范围的其余元素进行加标回收实验,其加标回收率为89.9%~117%。针对实际血清样品,通过优化稀释浓度和梯度,使用0.1 mL血清样品即可实现含量差异大的多种矿物质元素的测定。所建方法安全、高效、准确,可应用于大样本量人群样本的测定。A rapid method for the determination of more than 40 mineral elements in human serum was established.Serum samples were pretreated by direct dilution method,the diluent was com⁃posed of 0.01%Triton X-100 and 0.1%nitric acid,and then determined by inductively coupled plasma-mass spectrometry(ICP-MS)kinetic energy discrimination(KED)collision mode.The inter⁃nal standard method and the external standard method were used for methodological investigation.Un⁃der the selected instrument operating conditions,the internal standard method and the external stan⁃dard method had good linear relationships(r>0.999),and the detection limit was 0.000200-18.6μg/L.In the accuracy test,the results of 15 mineral elements of serum standards L-1 and L-2 given by the internal standard method and the external standard method were all within the recognized range.The recovery test was carried out for the remaining elements that did not give the identified range,the recovery was 89.9%-117%.For actual serum samples,by optimizing dilution concentra⁃tion and gradient,a wide range of mineral elements can be measured using only 0.1 mL of serum sample.This method is safe,efficient and accurate,and can be applied to the determination of large population samples.

关 键 词：电感耦合等离子体质谱法 人血清 矿物质元素 直接稀释 

分 类 号：O657.63[理学—分析化学] R977.5[理学—化学]