十全大补丸缓解顺铂诱导氧化应激肌萎缩的效果研究  

The Effect of Shiquan Dabu Pill (十全大补丸) on Alleviating Cisplatin-Induced Oxidative Stress Muscle Atrophy

作　　者：池梦怡 张聪颖 张楠楠 高晓艳 曲成谦 CHI Mengyi;ZHANG Congying;ZHANG Nannan;GAO Xiaoyan;QU Chengqian(School of Basic Medical Sciences,Chifeng University,Chifeng Inner Mongolia 024000,China)

机构地区：[1]赤峰学院基础医学院,内蒙古赤峰024000

出　　处：《中医药导报》2025年第1期42-47,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:探索十全大补丸缓解顺铂诱导氧化应激肌萎缩的效果。方法:将40只BALB/c小鼠随机分为空白对照组(NC组)、模型组(DDP组)、十全大补丸低剂量组[DDP+SQ(L)组]、十全大补丸中剂量组[DDP+SQ(M)组]及十全大补丸高剂量组[DDP+SQ(H)组],各8只。除NC组外,其余4组均腹腔注射给药4次顺铂(DDP,4 mg/kg,每3 d给药1次)。DDP+SQ(L)组、DDP+SQ(M)组、DDP+SQ(H)组每日灌胃给药相应剂量十全大补丸,共给药12 d。记录给药期间体质量和摄食变化,在实验最后一天测定各组小鼠前肢拉力,观察附睾脂肪、后肢及腓肠肌大小。通过苏木精-伊红(HE)染色法,观察并测定各组小鼠腓肠肌横截面积(CAS)。取肌肉组织用试剂盒检测总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化酶(CAT)、丙二醛(MDA)含量,检测肌肉组织活性氧(ROS)水平。结果:DDP可导致小鼠出现体质量下降、摄食减少、前肢拉力减弱、肌肉萎缩等表现,小鼠肌肉组织ROS增多,总抗氧化能力T-AOC下降,抗氧化相关酶SOD和CAT减少,氧化性物质MDA增多。使用十全大补丸治疗后的小鼠体质量及摄食增加,前肢拉力趋于空白对照组,CAS增加,肌肉萎缩情况得到改善,同时肌肉组织的T-AOC、SOD和CAT含量上升,MDA和ROS含量下降,趋于空白对照组。结论:十全大补丸具有缓解顺铂诱导氧化应激肌萎缩的效果。Objective:To explore the effect of Shiquan Dabu pill on alleviating cisplatin-induced oxidative stress muscle atrophy.Methods:Totally 40 BALB/c mice were randomly divided into blank control group(NC group),model group(DDP group),low-dose Shiquan Dabu pill grpup[DDP+SQ(L)group],medium-dose Shiquan Dabu pill group[DDP+SQ(M)group]and high-dose Shiquan Dabu pill group[DDP+SQ(H)group],8 mice in each group.Except for the NC group,the other 4 groups were administered 4 times of intraperitoneal injection of cisplatin(DDP,4 mg/kg,once every 3 days).DDP+SQ(L)group,DDP+SQ(M)group and DDP+SQ(H)group were administered the corresponding dose of Shiquan Dabu pill by gavage daily for a total of 12 days.The changes of body weight and food intake during administration were recorded,and the forelimb tensile force of mice in each group was measured on the last day of the experiment.The size of epididymal fat,hind limb and gastrocnemius muscle was observed.The cross-sectional area of gastrocnemius muscle(CAS)in each group of mice was observed and determined by hematoxylin-eosin(HE)staining.The total antioxidant capacity(T-AOC),superoxide dismutase(SOD),peroxidase(CAT)and malondialdehyde(MDA)levels were detected in muscle tissue,and the level of reactive oxygen species(ROS)in muscle tissue was detected.Results:DDP could lead to weight loss,reduced food intake,weakened forelimb tension,and muscle atrophy in mice,resulting in increased ROS,decreased T-AOC,decreased SOD and CAT,and increased MDA.After the treatment with Shiquan Dabu pill,the body weight and food intake increased,and the forelimb tension tended to be the normal group.The CAS increased,and the muscle atrophy was improved.The T-AOC,SOD,and CAT contents of muscle tissue increased,and the contents of MDA and ROS decreased,tending to be the normal group.Conclusion:Shiquan Dabu pill has the effect on alleviating cisplatin-induced oxidative stress muscle atrophy.

关 键 词：肌萎缩 顺铂 十全大补丸 氧化应激 小鼠 

分 类 号：R285.5[医药卫生—中药学]