Curcumin attenuates ochratoxin A and hypoxia co‑induced liver injury in grass carp(Ctenopharyngodon idella)by dual targeting endoplasmic reticulum stress and apoptosis via reducing ROS content  

作　　者：Liangqin Wu Piao Zhao Pei Wu Weidan Jiang Yang Liu Hongmei Ren Xiaowan Jin Xiaoqiu Zhou Lin Feng 

机构地区：[1]Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,China [2]Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China [3]Key Laboratory of Animal Disease-Resistance Nutrition,Ministry of Education,Ministry of Agriculture and Rural Affairs,Key Laboratory of Sichuan Province,Chengdu,Sichuan 611130,China

出　　处：《Journal of Animal Science and Biotechnology》2025年第1期408-423,共16页畜牧与生物技术杂志(英文版)

基　　金：This research was financially supported by the earmarked fund for CARS(CARS-45);National Natural Science Foundation of China(32273144,32072985);National Key R&D Program of China(2019YFD0900200).

摘　　要：Background Ochratoxin A(OTA)is a toxin widely found in aquafeed ingredients,and hypoxia is a common prob-lem in fish farming.In practice,aquatic animals tend to be more sensitive to hypoxia while feeds are contaminated with OTA,but no studies exist in this area.This research investigated the multiple biotoxicities of OTA and hypoxia combined on the liver of grass carp and explored the mitigating effect of curcumin(CUR).Methods A total of 720 healthy juvenile grass carp(11.06±0.05 g)were selected and assigned randomly to 4 experi-mental groups:control group(without OTA and CUR),1.2 mg/kg OTA group,400 mg/kg CUR group,and 1.2 mg/kg OTA+400 mg/kg CUR group with three replicates each for 60 d.Subsequently,32 fish were selected,divided into nor-moxia(18 fish)and hypoxia(18 fish)groups,and subjected to hypoxia stress for 96 h.Results CUR can attenuate histopathological damage caused by coming to OTA and hypoxia by reducing vacu-olation and nuclear excursion.The alleviation of this damage was associated with the attenuation of apoptosis in the mitochondrial pathway by decreasing the expression of the pro-apoptotic proteins Caspase 3,8,9,Bax,and Apaf1 while increasing the expression of the anti-apoptotic protein Bcl-2,and attenuation of endoplasmic reticulum stress(ERS)by reducing Grp78 expression and chop levels.This may be attributed to the fact that the addi-tion of CUR increased the levels of catalase(CAT)and glutathione reductase(GSH),increased antioxidant capacity,and ensured the proper functioning of respiratory chain complexes I and II,which in turn reduced the high produc-tion of reactive oxygen species(ROS),thus alleviating apoptosis and ERS.Conclusions In conclusion,our data demonstrate the effectiveness of CUR in attenuating liver injury caused by the combination of OTA and hypoxia.This study confirms the feasibility and efficacy of adding natural products to mitigate toxic damage to aquatic animals.

关 键 词：APOPTOSIS CURCUMIN Endoplasmic reticulum stress Grass carp HYPOXIA Ochratoxin A Oxidative damage 

分 类 号：S963[农业科学—水产养殖]