Th17细胞对MPTP诱导的帕金森病模型小鼠神经退变和神经炎症的影响  

Effects of Th17 cells on neurodegeneration and neuroinflammation in MPTP-induced Parkinson's disease model mice

作　　者：张倩[1] 王新 ZHANG Qian;WANG Xin(Department of Basic Medicine,Suzhou Vocational Health College,Suzhou 215009,China;不详)

机构地区：[1]苏州卫生职业技术学院基础医学部,江苏苏州215009 [2]苏州市中医院病理科,江苏苏州215009

出　　处：《医师在线》2025年第1期3-6,共4页Journal of Doctors Online

基　　金：苏州卫生职业技术学院科技项目课题(szwzy202204)。

摘　　要：目的探究辅助性T细胞17(Th17)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)模型小鼠神经退变及神经炎症的影响。方法选取C57BL/6小鼠48只,随机分为三组,腹腔注射生理盐水制备对照组,腹腔注射MPTP制备PD模型组,尾静脉注射Th17细胞的PD模型小鼠作为Th17组,每组16只。分别检测各组小鼠的行为学变化;免疫组织化学法检测小鼠中脑黑质的渗漏情况;免疫组织化学法和Western Blot法检测小鼠中脑黑质部位酪氨酸羟化酶(TH)阳性神经元数目变化及TH蛋白表达情况。结果PD模型组小鼠中脑黑质部位的荧光渗漏表现最明显;进一步观察发现,PD模型组小鼠纹状体部位有少量的荧光渗漏,皮质部位没有明显的荧光渗漏;对照组与PD模型组荧光渗漏区域相对荧光强度比较,差异有统计学意义(P<0.01)。在旷场实验中,与对照组相比,PD模型组小鼠移动距离、跨格数目减少,平均速度降低;Th17组小鼠移动距离、跨格数目更少,平均速度更低。在转棒实验中,与对照组相比,PD模型组小鼠在转棒上的潜伏时间缩短,Th17组小鼠的潜伏时间进一步缩短。与对照组相比,PD模型组小鼠中脑黑质部位TH阳性神经元数目、TH蛋白表达减少,Th17组小鼠减少更明显。结论Th17细胞及其炎症因子可以加重PD小鼠的神经炎症反应。Objective To investigate the effects of helper T cell 17(Th17)on neurodegeneration and neuroinflammation in Parkinson's disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods A total of 48 C57BL/6 mice were randomly divided into 3 groups.The control group was injected intraperitoneally with saline,the PD model group was injected intraperitoneally with MPTP,and the Th17 group consisting of PD model mice was injected with Th17 cells via the tail vein,with 16 mice in each group.The behavioral changes of mice in each group were detected respectively.The leakage in the substantia nigra of mice was detected by immunohistochemistry.The number of tyrosine hydroxylase(TH)positive neurons and the expression of TH protein in the substantia nigra of mice were detected by immunohistochemistry and Western Blot.Results The fluorescence leakage in the substantia nigra of the PD model group was the most obvious.Further observation showed that there was a small amount of fluorescence leakage in the striatum of the PD model group,and there was no obvious fluorescence leakage in the cortex.There was a statistically significant difference in the relative fluorescence intensity of the fluorescence leakage area between the control group and the PD model group(P<0.01).In the open-field test,compared with the control group,the moving distance,the number of crossing grids and the average speed of the mice in the PD model group decreased.The mice in the Th17 group had less moving distance,less number of cross grids,and lower average speed.In the rota-rod test,compared with the control group,the latency time of mice in the PD model group was shortened,and the latency time of mice in the Th17 group was further shortened.Compared with the control group,the number of TH positive neurons and the expression of TH protein in the substantia nigra of the PD model group were decreased,and the mice in the Th17 group were further reduced.Conclusion Th17 cells and their inflammatory factors can exacerbate the neuroi

关 键 词：帕金森病 TH17 神经炎症 

分 类 号：R74[医药卫生—神经病学与精神病学]