Visfatin(NAMPT)affects global gene expression in porcine anterior pituitary cells during the mid-luteal phase of the oestrous cycle  

作　　者：Kamil Dobrzyn Grzegorz Kopij Marta Kiezun Ewa Zaobidna Marlena Gudelska Barbara Zarzecka Lukasz Paukszto Agnieszka Rak Nina Smolinska Tadeusz Kaminski 

机构地区：[1]Faculty of Biology and Biotechnology,University of Warmia and Mazury in Olsztyn,Olsztyn,Poland [2]School of Medicine,Collegium Medicum,University of Warmia and Mazury in Olsztyn,Olsztyn,Poland [3]Institute of Zoology and Biomedical Research,Jagiellonian University,Krakow,Poland

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2293-2315,共23页畜牧与生物技术杂志(英文版)

基　　金：supported by the Polish National Science Center research grants:OPUS 16[grant number:2018/31/B/NZ9/00781];OPUS 20[grant number:2020/39/B/NZ9/01061]and SONATA 16[grant number:2020/39/D/NZ9/01009]。

摘　　要：Background The pituitary belongs to the most important endocrine glands involved in regulating reproductive functions.The proper functioning of this gland ensures the undisturbed course of the oestrous cycle and affects the female's reproductive potential.It is believed that visfatin,a hormone belonging to the adipokine family,may regulate reproductive functions in response to the female's metabolic state.Herein we ve rified the hypothesis that suggests a modulatory effect of visfatin on the anterior pituitary transcriptome during the mid-luteal phase of the oestrous cycle.Results RNA-seq analysis of the porcine anterior pituitary cells revealed changes in the expression of 202 genes(95 up-regulated and 107 down-regulated in the presence of visfatin,when compared to the non-treated controls),assigned to 318 gene ontology terms.We revealed changes in the frequency of alternative splicing events(235 cases),as well as long noncoding RNA expression(79 cases)in the presence of the adipokine.The identified genes were associated,among others,with reproductive system development,epithelial cell proliferation,positive regulation of cell development,gland morphogenesis and cell chemotaxis.Conclusions The obtained results indicate a modulatory influence of visfatin on the regulation of the porcine transcriptome and,in consequence,pituitary physiology during the mid-luteal phase of the oestrous cycle.

关 键 词：Alternative splicing Anterior pituitary Differentially expressed genes lncRNA Oestrous cycle Pig RNASEQ TRANSCRIPTOME VISFATIN 

分 类 号：S828[农业科学—畜牧学]