The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oo ytes potentially by promoting histone lactylation  

作　　者：Yaozong Wei Bo Pan Jianpeng Qin Beijia Cao Tianyi Lv Jiangfeng Ye Ao Ning Kunlin Du Xiangyi Chen Shuqi Zou Shengqin Zang Guozhi Yu Tianzeng Song Qiuxia Liang Guangbin Zhou 

机构地区：[1]State Key Laboratory of Swine and Poultry Breeding Industry,Key Laboratory of Livestock and Poultry Multi‑Omics,Ministry of Agriculture and Rural Affairs,and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China [2]College of Life Science,Sichuan Agricultural University,Ya’an 625014,Sichuan,China [3]Institute of Animal Science,Xizang Academy of Agricultural and Animal Husbandry Science,Lhasa 850009,Xizang,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2333-2350,共18页畜牧与生物技术杂志(英文版)

基　　金：supported by the National Key Research and Development Program of China(No.2021YFD1200403);the National Natural Science Foundation of China(No.32072735);Xigaze City regional Science and technology Collaborative innovation project-Genetic resources of rescue protection for Zhangmu goat(No.QYXTZX-RKZ2023-06)。

摘　　要：Background Previous studies have shown that the vitrification of metaphaseⅡ(MⅡ)oocytes significantly represses their developmental potential.Abnormally increased oxidative stress is the probable factor;however,the underlying mechanism remains unclear.The walnut-derived peptide TW-7 was initially isolated and purified from walnut protein hydrolysate.Accumulating evidences implied thatTW-7 was a powerful antioxidant,while its prospective application in oocyte cryopreservation has not been reported.Result Here,we found that parthenogenetic activation(PA)zygotes derived from vitrified MⅡoocytes showed elevated ROS level and delayed progression of pronucleus formation.Addition of 25μmol/LTW-7 in warming,recovery,PA,and embryo culture medium could alleviate oxidative stress in PA zygotes from vitrified mouse MⅡoocytes,furtherly increase proteins related to histone lactylation such as LDHAe LDHB,and EP300 and finally improve histone lactylation in PA zygotes.The elevated histone lactylation facilitated the expression of minor zygotic genome activation(ZGA)genes and preimplantation embryo development.Conclusions Our findings revealed the mechanism of oxidative stress inducing repressed development of PA embryos from vitrified mouse MⅡoocytes and found a potent and easy-obtained short peptide that could significantly rescue the decreased developmental potential of vitrified oocytes,which would potentially contribute to reproductive medicine,animal protection,and breeding.

关 键 词：Histone lactylation OOCYTE TW-7 VITRIFICATION Zygotic genome activation 

分 类 号：S814.8[农业科学—畜牧学]