沙棘籽油的超临界CO_(2)提取及抗氧化活性研究  

Supercritical CO_(2)Extraction and Antioxidant Activity of Sea Buckthorn Seed Oil

作　　者：刘美静 魏晓博 余君伟 虎丽 方海田[1] 刘慧燕 Liu Meijing;Wei Xiaobo;Yu Junwei;Hu Li;Fang Haitian;Liu Huiyan(College of Food Science and Engineering,Ningxia University,Yinchuan 750021;Ningxia Zhongning Wolfberry Industry Innovation Research Institute,Zhongning 755100;Ningxia Veterinary Drug Feed Supervision Institute,Yinchuan 750021)

机构地区：[1]宁夏大学食品科学与工程学院,银川750021 [2]宁夏中宁枸杞产业创新研究院,中宁755100 [3]宁夏兽药饲料监察所,银川750021

出　　处：《中国粮油学报》2025年第1期156-161,共6页Journal of the Chinese Cereals and Oils Association

摘　　要：探究“药食同源”沙棘籽油的营养保健功效及抗氧化活性,为沙棘籽油的开发利用提供参考。采用超临界CO_(2)技术萃取沙棘籽油,测定基础理化指标和天然生物活性成分,利用高效气相色谱法分析沙棘籽油的脂肪酸组成和水平,通过自由基清除实验等评价其体外抗氧化活性。结果表明,超临界萃取技术萃取沙棘籽油平均得率为10.83%,所得沙棘籽油各项理化指标符合行业标准;经高效气相色谱仪共检测出18种脂肪酸,其中,总不饱和脂肪酸质量分数达88.12%,以油酸(23.11%)、亚油酸(35.60%)和亚麻酸(28.91%)为主;总黄酮含量为27.15 mg RE/100 g,总酚含量为41.60 mg GAE/100 g;清除DPPH、ABTS和羟基自由基的IC 50值分别为3.82、16.17、0.50 mg/mL,表现出较强的抗氧化能力。超临界CO_(2)技术萃取的沙棘籽油不饱和脂肪酸和天然生物活性成分含量丰富,有较强体外抗氧化活性,具有潜在利用价值。The nutritional and health effects and antioxidant activity of medicinal and edible sea buckthorn seed oil were explored to provide reference for the development and utilization of sea buckthorn seed oil.The sea buckthorn seed oil was extracted by supercritical CO_(2)technology,and the basic physical and chemical indexes and natural bioactive components were determined.The fatty acid composition and level of sea buckthorn seed oil were analyzed by high performance gas chromatography,and its antioxidant activity in vitro was evaluated by free radical scavenging experiment.The results indicated that the average yield of sea buckthorn seed oil extracted by supercritical extraction technology was 10.83%,and the physical and chemical indexes of sea buckthorn seed oil were in line with the industry standard.A total of 18 fatty acids were detected by high performance gas chromatography.Among which,the total unsaturated fatty acid content was 88.12%,mainly oleic acid(23.11%),linoleic acid(35.60%)and linolenic acid(28.91%).The content of total flavonoids was 27.15 mg RE/100 g,and the content of total phenols was 41.60 mgGAE/100 g.The IC 50 values of scavenging DPPH,ABTS and hydroxyl radicals were 3.82,16.17 and 0.50 mg/mL,respectively,showing strong antioxidant capacity.In summary,the sea buckthorn seed oil extracted by supercritical CO_(2)technology was rich in unsaturated fatty acids and natural bioactive components,and had strong in vitro antioxidant activity and potential use value.

关 键 词：沙棘籽油 超临界CO_(2)萃取 脂肪酸 生物活性成分 抗氧化 

分 类 号：TS222.1[轻工技术与工程—粮食、油脂及植物蛋白工程]