基于16SrDNA测序研究瘀浊脂消丸对非酒精性脂肪性肝炎大鼠肠道菌群的影响  

Effects of Yuzhu Zhixiao Pill on intestinal flora of rats with nonalcoholic steatohepatitis based on 16S rDNA sequencing

作　　者：杜沅沁 徐健 黄鸿娜[2] 黄晶晶[2,3] 农耀斌 钟瑞熙 朱荣火 谭钦文 贾菊红 毛德文 黄贵华[2] DU Yuanqin;XU Jian;HUANG Hongna;HUANG Jingjing;NONG Yaobin;ZHONG Ruixi;ZHU Ronghuo;TAN Qinwen;JIA Juhong;MAO Dewen;HUANG Guihua(Guangxi University of Chinese Medicine,Nanning 530000,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China;Guangxi Key Laboratory of Translational Medicine for Treating High-incidence Infectious Diseases with Integrative Medicine,Nanning 530000,China)

机构地区：[1]广西中医药大学,南宁530000 [2]广西中医药大学第一附属医院,南宁530023 [3]广西高发传染病中西医结合转化医学重点实验室,南宁530000

出　　处：《中华中医药杂志》2024年第12期6683-6689,共7页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西自然科学基金面上项目(No.2022GXNSFAA035460);广西自然科学基金青年科学基金项目(No.2022GXNSFBA035485);广西名中医传承工作室建设项目(No.桂卫中医发[2017]2号);桂派中医大师培养项目(No.桂中医药科教发[2022]6号);广西中医药大学研究生教育创新计划项目(No.YCSY2023021);广西中医药大学引进博士科研启动基金项目(No.2022BS026)。

摘　　要：目的:观察瘀浊脂消丸对高脂饲料诱导的非酒精性脂肪性肝炎(NASH)大鼠的治疗作用,并基于肠道菌群变化探讨其作用机制。方法:将40只雄性SD大鼠随机均分为空白(Con)组、模型(MOD)组、瘀浊脂消丸(YZZXP)组和双歧杆菌(Prob)组。高脂饮食喂养12周建立大鼠NASH模型。给药12周后检测大鼠肝功能及血脂各项指标;取结肠内容物做16S rDNA检测;取肝组织进行HE染色和油红O染色,并且检测其甘油三酯(TG)含量。结果:与Con组比较,MOD组大鼠丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总胆汁酸(TBA)、总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)显著升高(P<0.05),而高密度脂蛋白胆固醇(HDL-C)显著降低(P<0.05),肝脏病理形态恶化,TG和NAS评分显著升高(P<0.05),Oscillospira和Roseburia等8个菌属显著上调(P<0.05),Bifidobacterium和Lachnospira等6个菌属显著下调(P<0.05);与MOD组比较,YZZXP组ALT、AST、ALP、TBA、TC、TG和LDL-C显著降低(P<0.05),而HDL-C显著升高(P<0.05),肝脏病理形态好转,Akkermansia和Turicibacter等8个菌属显著上调(P<0.05),Ralstonia和Paraprevotella等10个菌属显著下调(P<0.05)。结论:瘀浊脂消丸可以抑制Roseburia等有害菌属繁衍,增加Akkermansia等有益菌属来调节代谢途径,调控血脂治疗高脂饮食诱导的NASH。Objective:To observe the therapeutic effect of Yuzhuo Zhixiao Pill on rats with nonalcoholic steatohepatitis(NASH)induced by high fat diet,and to explore its mechanism based on the changes of intestinal flora.Methods:A total of 40 male SD rats were randomly divided into blank(Con)group,model(MOD)group,Yuzhuo Zhixiao Pill(YZZXP)group and bifidobacterium(Prob)group.NASH model was established after 12 weeks of high fat diet.The indexes of liver function and blood lipid were detected after 12 weeks of administration.Colon contents were collected for 16S rDNA detection.Liver tissue was stained with HE and oil red O,and triglyceride(TG)content was detected.Results:Compared with Con group,ALT,AST,ALP,TBA,TC,TG and LDL-C in the MOD group were significantly increased(P<0.05),while HDL-C in the MOD group was decreased(P<0.05),liver pathological morphology was deteriorated,TG and NAS score were significantly increased(P<0.05),and 8 bacteria genera such as Oscillospira and Roseburia were significantly up-regulated in MOD group(P<0.05),Bifidobacterium and Lachnospira were significantly down-regulated in MOD group(P<0.05).Compared with MOD group,ALT,AST,ALP,TBA,TC,TG and LDL-C in the YZZXP group were significantly decreased(P<0.05),while HDL-C in the YZZXP group was increased(P<0.05),YZZXP group improved liver pathological morphology,and significantly up-regulated 8 bacteria genus such as Akkermansia and Turicibacter(P<0.05),Ralstonia and Paraprevotella were significantly down-regulated(P<0.05).Conclusion:Yuzhuo Zhixiao Pill can inhibit the proliferation of harmful bacteria such as Roseburia,increase the beneficial bacteria such as Akkermansia,regulate metabolic pathway,regulate blood lipid in the treatment of high-fat diet-induced NASH.

关 键 词：非酒精性脂肪性肝炎 瘀浊脂消丸 16S rDNA 肠道菌群 机制 

分 类 号：R57[医药卫生—消化系统]