SCYL1在小鼠急性肝衰竭中表达及意义  

Expression and significance of SCYL1 in acute liver failure in mice

作　　者：叶向阳[1,2] 林珑 欧寒冰 何雄志 胡震霆[1] 郑烽锋[1] 黄晓刚[1] 谢雪梅[1] 陈妃花 邱荣仙 YE Xiangyang;LIN Long;OU Hanbing;HE Xiongzhi;HU Zhenting;ZHENG Fengfeng;HUANG Xiaogang;XIE Xuemei;CHEN Feihua;QIU Rongxian(Department of Infectious Diseases,the Affiliated Hospital of Putian University,Putian,Fujian 351100,China;Department of Clinical Medicine,Fujian Medical University,Fuzhou,Fujian 350122,China;College of Humanities and Management,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China)

机构地区：[1]莆田学院附属医院感染性疾病科,福建莆田351100 [2]福建医科大学临床医学部,福建福州350122 [3]福建中医药大学人文与管理学院,福建福州350122

出　　处：《热带医学杂志》2024年第12期1689-1692,1704,I0001,共6页Journal of Tropical Medicine

基　　金：福建省中青年教师教育科研项目(JAT220290)。

摘　　要：目的探讨SCY1样假激酶1(SCYL1)在急性肝衰竭中的表达及作用机制,为急性肝衰竭患者的诊断与治疗提供新思路。方法12只SPF级BALB/c雄性小鼠随机分为对照组(n=6)和肝衰竭组(n=6),肝衰竭组采用D-半乳糖胺盐酸盐溶液(32 mg/mL,0.5 mL/只)注射小鼠腹腔构建急性肝衰竭模型,对照组不做干预。造模结束后,苏木精-伊红(HE)染色观察肝组织病变情况,生化检测血清中的丙氨酸氨基转移酶(ALT)含量,酶联免疫吸附法(ELISA)检测白细胞介素-10(IL⁃10)和肿瘤坏死因子-α(TNF⁃α)的含量,通过实时荧光定量PCR(RT⁃qPCR)检测肝组织中SCYL1和高尔基蛋白73(GP73)相对表达量。基因本体(GO)和京都基因与基因组百科全书(KEGG)数据库分析SCYL1富集通路。结果HE结果显示,对照组肝脏表面光滑、肝细胞完整且胞质丰富、肝小叶结构清晰;肝衰竭组表现出细胞肿胀、坏死、炎性细胞浸润、双核肝细胞增多、多个假小叶形成。与对照组比较,肝衰竭组血清ALT、TNF⁃α含量升高,IL⁃10含量降低,差异均有统计学意义(t=-7.260、-17.126、11.349,P均<0.05)。RT⁃qPCR结果显示,肝衰竭组小鼠肝组织中SCYL1、GP73相对表达量[(1.51±0.22),(2.66±0.56)]高于对照组[(1.25±0.18),(1.40±0.24)],差异均有统计学意义(t=2.734、-1.820,P均<0.05)。KEGG和GO数据库分析显示,SCYL1参与了内质网-高尔基体-囊泡转运相关通路。结论SCYL1在肝组织的异常表达,可能通过调节高尔基体转运及神经元细胞的正常发育促进肝衰竭及并发症的发生发展。Objective To investigate the expression and significance of SCY1⁃like pseudokinase 1(SCYL1)in patients with acute liver failure,and provide new ideas for the diagnosis and treatment of acute liver failure.Methods Twelve mice were randomly divided into control group(n=6)and liver failure group(n=6).The liver failure group was injected into the abdominal cavity of mice with D⁃galactosamine hydrochloride solution(32 mg/mL,0.5 mL/mouse)to establish mouse acute liver failure model,while the control group was not interfered.After the modeling,the liver tissue lesions were observed by hematoxylin⁃eosin(HE)staining,the serum alanine aminotransferase(ALT)content was detected by biochemical assay,the contents of interleukin⁃10(IL⁃10)and tumor necrosis factor⁃α(TNF⁃α)were detected by enzyme⁃linked immunosorbent assay(ELISA),and the relative expression levels of SCYL1 and Golgi protein 73(GP73)were detected by real⁃time fluorescence quantitative PCR(RT⁃qPCR).Gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG)databases were used to analyze the SCYL1 enrichment pathway.Results HE staining results showed that the control group showed smooth liver surface,intact hepatocytes with abundant cytoplasm and clear structure of hepatic lobules;while the liver failure group showed cell swelling,necrosis,inflammatory cell infiltration,double nucleated hepatocytes increase,and formation of multiple pseudolobules.Compared with the control group,the levels of ALT and TNF⁃αin serum of liver failure group were increased while the levels of IL⁃10 were decreased,with statistical significance(t=-7.260,-17.126,11.349;all P<0.05).The RT⁃qPCR results showed that the relative expression levels of SCYL1 and GP73 in the liver tissues of liver failure group[(1.51±0.22),(2.66±0.56)]was significantly higher than those in the control group[(1.25±0.18),(1.40±0.24)](t=2.734,-1.820;both P<0.05).KEGG and GO database analysis showed that SCYL1 was involved in endoplasmic reticulum⁃Golgi⁃vesicle transport⁃relate

关 键 词：急性肝衰竭 SCY1样假激酶1 高尔基蛋白73 白细胞介素-10 

分 类 号：R575.3[医药卫生—消化系统]