一种具有抑菌性的pH响应新型水凝胶的制备及性能  

Preparation and characterization of a new pH-responsive hydrogel with bacteriostatic properties

作　　者：岳灿灿 赵鸿鹏 刘文森 许娜[3] 张淑华[1] 郗艳丽[3] YUE Cancan;ZHAO Hongpeng;LIU Wensen;XU Na;ZHANG Shuhua;XI Yanli(School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022,China;Changchun Institute of Veterinary Medicine,Chinese Agricultural Sciences,Changchun 130122,China;School of Public Health,Jilin Medical University,Jilin 132013,China)

机构地区：[1]长春理工大学生命科学与技术学院,长春130022 [2]中国农业科学院长春兽医研究所,长春130122 [3]吉林医药学院,吉林132013

出　　处：《复合材料学报》2024年第12期6702-6712,共11页Acta Materiae Compositae Sinica

基　　金：吉林省科技厅科技发展计划项目(20220204127YY);吉林省科技厅重点研发项目(20220204035YY)。

摘　　要：为设计一种能够响应pH的、新型多功能创面敷料,使用季铵化壳聚糖(QCS)、接枝了磺胺嘧啶(SD)的氧化葡聚糖(Dex-CHO/SD)和妥布霉素(TOB),通过席夫碱键和氢键反应形成DSTQ水凝胶。对水凝胶的形貌、降解性、抑菌性、细胞相容性、血液相容性等性能进行研究。结果表明:具有三维孔状结构的DSTQ水凝胶可以响应感染造成的pH降低,进而发生降解,释放药物;DSTQ水凝胶对铜绿假单胞菌的抑制率显著高于金黄色葡萄球菌;DSTQ水凝胶毒性低,生物安全性高,与L929细胞共培养3 d后,细胞活力高于95%;DSTQ水凝胶具有良好的血液相容性,溶血率远小于5%;细胞在DSTQ水凝胶表面能够快速迁移,有利于伤口愈合;当DSTQ水凝胶负载生长因子后具有良好的抗炎效果。研究结果表明,DSTQ水凝胶可以作为新型创面敷料,有望应用于临床促伤口愈合。To develop a novel wound dressing with multiple functionalities and pH-responsiveness,a combination of quaternary ammonium chitosan(QCS),oxidized dextran grafted with sulfadiazine(Dex-CHO/SD),and tobramycin(TOB) was employed to fabricate the Dex-CHO/SD/TOB(DSTQ) hydrogel through Schiff base bonding and hydrogen bonding.The hydrogel formation relied on Schiff base bonding and hydrogen bonding.The morphology,degradation,bacteriostatic,cytocompatibility,and hemocompatibility properties of the hydrogels were analyzed.The results indicated that the 3D pore structure of DSTQ hydrogel could respond to pH drops caused by infections and degrade,facilitating drug release.The inhibition rate of DSTQ hydrogel against Pseudomonas aeruginosa is significantly higher than that of Staphylococcus aureus.DSTQ hydrogel exhibits low toxicity,high biosafety,and cell viability above 95% after three days of co-culture with L929 cells.Additionally,it demonstrates excellent hemocompatibility,with a hemolysis rate below 5%.Furthermore,cells exhibit rapid migration on the material's surface,promoting wound healing.When loaded with growth factors,DSTQ hydrogel demonstrates good anti-inflammatory effects.These findings suggest that DSTQ hydrogel can be an effective wound-dressing option in clinical settings.

关 键 词：水凝胶 伤口愈合 PH响应性 抑菌性 伤口敷料 

分 类 号：TB332[一般工业技术—材料科学与工程]