银叶树树叶三萜类成分的提取工艺及其抗氧化活性研究  

Extraction process and Antioxidant Activity of Triterpenoids from the Leaves of Heritiera littoralis Dryand

作　　者：皮睿 张艳军 杨历 邓勤 尹艳镇 PI Rui;ZHANG Yan-jun;YANG Li;DENG Qin;YIN Yan-zhen(College of Petroleum and Chemical Engineering,Beibu Gulf University,Qinzhou 535011,China;Guangxi Key Laboratory of Green Chemical Materials and Safety Technology,Qinzhou 535000,China)

机构地区：[1]北部湾大学石油与化工学院,广西钦州535011 [2]广西绿色化工与新材料安全技术重点实验室,广西钦州535000

出　　处：《化学试剂》2025年第2期46-54,共9页Chemical Reagents

基　　金：药用红树林植物银叶树中萜类化合物的抗炎活性及其对NF-KB通路影响的应用基础研究项目(2021GXNSFBA220100);基于OMAC策略从广西红树林植物内生真菌代谢产物中发现抗炎活性成分的研究项目(2021KYQD05)。

摘　　要：为研究药用红树林植物银叶树树叶中三萜类成分的提取工艺及其抗氧化活性,以该植物树叶为原材料,探究超声提取功率、乙醇浓度、料液比、超声提取时间4个单因素对提取率的影响;在单因素实验的基础上,采用正交试验优化提取工艺;利用大孔树脂对提取所得三萜进行纯化,高效液相色谱法分析纯化前后样品中桦木酸与齐墩果酸的含量;最后对纯化三萜进行抗氧化测定。实验结果表明,最佳工艺条件为:超声提取功率240 W、乙醇浓度80%、料液比1∶70(g/mL)、超声时间25 min,此条件下的银叶树三萜类成分提取率为9.17%;大孔树脂纯化后的银叶树三萜纯度较纯化前提高了1.69倍;纯化前桦木酸含量为0.942 mg/g,纯化后含量为3.471 mg/g;纯化前齐墩果酸未检出,纯化后含量0.412 mg/g。对纯化后的银叶树三萜类成分进行DPPH自由基清除实验、羟基自由基清除实验和还原能力实验,其对DPPH自由基的IC 50为8.08μg/mL,对羟基自由基IC 50为7.21μg/mL,且还原能力随浓度的升高而增强,表明银叶树三萜类具有较好的抗氧化活性。该研究将为银叶树三萜作为抗氧化剂进一步开发利用提供数据基础。To optimize the extraction process and evaluate the antioxidant activity in vitro of triterpenoids from the medicinal mangrove plant Heritiera littoralis Dryand,the leaves of H.littoralis as raw material were choosed.The influences of four single factors including ultrasonic extraction power,ethanol concentration,solid-liquid ratio,and ultrasonic extraction time on the extraction rates were explored.Orthogonal experiments were employed to optimize the extraction conditions obtained from the single-factor experiments.The macroporous resin was used to purify the extracted triterpenoids.The contents of betulinic acid and oleanolic acid in the samples before and after purification were analyzed by high performance liquid chromatography.Finally,the antioxidant activity of the purified triterpenoids was determined.The optimal process conditions for ultrasound-assisted extraction of H.littoralis triterpenoids were determined as follows:ultrasonic extraction power 240 W,ethanol concentration 80%,material-liquid ratio 1∶70(g/mL),and ultrasonic extraction time 25 min.The extraction rate of triterpenoids under the optimal extraction conditions was 9.17%.The purity of triterpenoids purified by macroporous resin was 1.69 times higher than that before purification.The contents of betulinic acid before and after purification were 0.942 and 3.471 mg/g,respectively.The oleanolic acid was not detected before purification and the content after purification was 0.412 mg/g.The DPPH radical scavenging experiment,hydroxyl radical scavenging experiment,and iron reduction ability experiment were conducted on the purified H.littoralis triterpenoids.The result showed that the IC 50 values of purified triterpenoids were 8.08μg/mL for DPPH and 7.21μg/mL for hydroxyl free radical.Moreover,the reducing capacity increased with the increase of concentration,indicating that H littoralis triterpenoids possessed good antioxidant activity.This study provides a data basis for the further development and utilization of H.littoralis triterpenoids a

关 键 词：银叶树 三萜 提取工艺 分离纯化 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]