Metabolomic profiling of cerebrospinal fluid in neurosyphilis:identification of potential diagnostic biomarkers  

神经梅毒脑脊液代谢组学分析:潜在诊断生物标记物的鉴定

作　　者：HE Yuanyuan WU Jiaxin CHEN Zhaoqi DENG Ziliang WANG Haiying KE Wujian 贺媛媛;吴嘉欣;陈钊琪;邓梓亮;王海鹰;柯吴坚(南方医科大学公共卫生学院,广东广州510515;南方医科大学皮肤病医院,广东广州510091)

机构地区：[1]School of Public Health,Southern Medical University,Guangzhou 510515,China [2]Dermatology Hospital,Southern Medical University,Guangzhou 510091,China

出　　处：《皮肤性病诊疗学杂志》2024年第12期801-809,共9页Journal of Diagnosis and Therapy on Dermato-venereology

摘　　要：Objective This study aims to analyze metabolites in the cerebrospinal fluid(CSF)of neurosyphilis patients to identify potential diagnostic markers.Methods Untargeted metabolomics by liquid chromatography-mass spectrometry was used to analyze metabolites in CSF samples from five neurosyphilis and five syphilis/non-neurosyphilis patients.After quality control and normalization,data analyses(principal component analysis,orthogonal partial least squares-discriminant analysis,hierarchical clustering)were conducted to identify differential metabolites.Results The content of metabolites(Pro-leu,nudifloramide,N-oleoyl glycine,DMP 777,(±)-propionylcarnitine,and 3β-hydroxy-5-cholenoic acid)in neurosyphilis patients was 1.7-to 4.5-fold of that in the non-neurosyphilis patients.Conclusions The metabolites,particularly nudifloramide and N-oleoyl glycine,which have been implicated in other neurological disorders,warrant further investigation into their roles in the pathogenesis of neurosyphilis.目的分析神经梅毒患者的脑脊液代谢物,以确定潜在的诊断标志物。方法利用液相色谱-质谱联用技术的非靶向代谢组学,分析5例神经梅毒患者和5例梅毒/非神经梅毒患者的脑脊液样本。将原始数据经过质量控制和归一化处理后,进行数据分析(主成分分析、正交偏最小二乘判别分析、层次聚类分析)确定两组间的差异代谢物。结果神经梅毒患者体内,有6种代谢物显著升高,分别是Pro-leu、nudifloramide、N-oleoyl glycine、DMP 777、(±)-propionylcarnitine和3β-hydroxy-5-cholenoic acid。这6种代谢物两组间的差异倍数范围是1.7-4.5。结论所发现的代谢物,特别是nudifloramide和N-oleoyl glycine,曾被报道与其他神经系统疾病有关,其在神经梅毒发病机制中的作用值得进一步研究。

关 键 词：NEUROSYPHILIS metabolomic profiling cerebrospinal fluid diagnostic biomarkers 

分 类 号：R73[医药卫生—肿瘤]