唾液ANG-2浓度和口腔扁平苔藓炎症活跃程度的关联性分析  

Correlation between salivary ANG-2 level and inflammation activity of oral lichen planus

作　　者：向远燚 罗晶晶[2] 李海洋 吴中婷[1,4] 周红梅 XIANG Yuanyi;LUO Jingjing;LI Haiyang;WU Zhongting;ZHOU Hongmei(State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Frontier Innovation Center for Dental Medicine Plus,Depart-ment of Oral Medicine,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China)

机构地区：[1]口腔疾病防治全国重点实验室,国家口腔医学中心,国家口腔疾病临床医学研究中心,“口腔医学+”前沿医学创新中心,四川大学华西口腔医院口腔黏膜病科,四川成都610041 [2]口腔疾病防治全国重点实验室,国家口腔医学中心,国家口腔疾病临床医学研究中心,“口腔医学+”前沿医学创新中心,四川大学华西口腔医院预防口腔科,四川成都610041 [3]重庆医科大学附属口腔医院大学城门诊部口腔科,重庆400015 [4]攀枝花市攀钢集团总医院口腔科,四川攀枝花617000

出　　处：《口腔医学》2025年第1期45-50,共6页Stomatology

基　　金：国家自然科学基金(81272962,82071124,82301094);四川大学华西口腔医院探索与研发项目(RD-03-202410)。

摘　　要：目的检测口腔扁平苔藓(oral lichen planus,OLP)患者的血管生成素-2(angiopoietin 2,ANG-2)唾液浓度,分析其与OLP炎症活跃程度的关联性。方法纳入89例OLP患者,分为无症状非糜烂型组、有症状非糜烂型组、轻中度糜烂型组及重度糜烂型组,15名健康成人作为正常对照组。收集受试者非刺激性全唾液,采用化学发光免疫法检测ANG-2浓度并进行分析。收集正常口腔黏膜组织和非糜烂型、糜烂型OLP口腔黏膜组织,采用免疫组化染色检测间质血管ANG-2表达。结果与正常对照组相比,OLP组年龄和性别均无统计学差异,OLP全组及非糜烂型、糜烂型OLP组的唾液ANG-2浓度均显著增高(P<0.05);其中,糜烂型OLP组显著高于非糜烂型(P=0.022),有症状非糜烂型组高于无症状非糜烂型组(P=0.048),糜烂型亚组之间无统计学差异(P=0.067)。Spearman相关性分析显示,唾液ANG-2浓度与OLP患者炎症活跃程度呈正相关(r=0.314,P=0.003)。非糜烂型OLP间质血管的ANG-2表达较正常黏膜组织增加,但无统计学意义(P>0.05);糜烂型OLP间质血管ANG-2表达较正常黏膜组织(P<0.001)及非糜烂型OLP(P<0.001)均显著增加。结论唾液ANG-2浓度与OLP炎症活跃程度有较高关联性,提示其可作为OLP炎症活跃标志物之一,辅助临床无创监测OLP病情变化。Objective To assay the salivary ANG-2 level of oral lichen planus(OLP)patients,and analyze its correlation with inflammatory activity of OLP.Methods Eighty-nine OLP patients were included,and divided into four subgroups as non-erosive asymptomatic(NEA),non-erosive symptomatic(NES),minor-erosive(MIE)and major-erosive(MAE)groups.Fifteen healthy adults were recruited as controls.Whole unstimulated saliva was collected from each participant,and the salivary ANG-2 level was measured by chemiluminescence immunoassays(CLIA)for analysis.Normal oral mucosal tissue,non-erosive and erosive OLP tissues were collected to detect and analyze the expression of ANG-2 positive blood vessels by immunohistochemistry(IHC).Results The base-lines of age and gender between OLP and control groups showed no significant difference.Compared to controls,the salivary ANG-2 levels of OLP group,non-erosive and erosive OLP subgroups were significantly higher(P<0.05),in which erosive OLP group was higher than non-erosive OLP group(P=0.022);NES subgroup was slightly higher than NEA(P=0.048),and there was no statistical significance between MIA and MEA subgroups(P=0.067).Spearman correlation analysis showed a positive correlation between salivary ANG-2 level and inflammation activity in OLP patients(r=0.314,P=0.003).The expression of ANG-2 in non-erosive OLP mucosal tissues slightly increased than normal oral mucosal tissue(P>0.05),but there was no significant difference.The expression of ANG-2 in erosive OLP mucosal tissues significantly increased than normal oral mucosal tissue(P<0.001)and non-erosive OLP group(P<0.001).Conclusion There is a certain correlation between sali-vary ANG-2 level and inflammatory activity of OLP,indicating that salivary ANG-2 level is probable to be one of the inflammatory activity indicators to monitor the state-variation of OLP as a clinical non-inva-sive method.

关 键 词：口腔扁平苔藓 炎症程度 血管生成素-2 唾液 无创监测 

分 类 号：R363.2[医药卫生—病理学]