香菇废菌棒诱导黑水虻肠道纤维素降解菌筛选  

Screening of Intestinal Cellulose-degrading Bacteria Induced by Shiitake Mushroom Sticks of Hermetia illucens

作　　者：覃春桃 余洪勤 王颖娟 QIN Chuntao;YU Hongqin;WANG Yingjuan(Key Laboratory of Research and Development of Ethnic Medicinal Biological Resources,Minzu Normal University of Xingyi,Xingyi,Guizhou 562400,China)

机构地区：[1]兴义民族师范学院民族药用生物资源研究与开发实验室,贵州兴义562400

出　　处：《农产品加工》2025年第1期68-70,共3页Farm Products Processing

基　　金：2022年国家级大学生创新创业项目“食用菌废菌棒的环境昆虫处理途径及资源化利用探讨”(202210666282);兴义民族师范学院教授基金项目“蚁蛉触角转录组及化学感受器相关基因研究”(19XYJS03);贵州省教育厅高校科技创新团队(黔教技[2023]095号);兴义民族师范学院科研创新团队“山地特色药食同源动物资源研究与开发科研创新团队”(23XYTD04);兴义民族师范学院学科建设项目“山地特色食药用菌病虫害调查及防治研究”(XKJS202310)。

摘　　要：通过用香菇废菌棒饲养黑水虻,诱导建立肠道菌群,利用羧甲基纤维素钠培养基和卢戈氏碘液染色法,分离筛选肠道高产纤维素酶菌株并测定滤纸酶活力。结果表明,从香菇废菌棒诱导黑水虻肠道菌群中,筛选出1株高产纤维素酶分解菌,其D/d值达4.17,滤纸酶活力为100.43 U/mL,显示具有较高的纤维素降解能力。The intestinal high-yielding cellulase strains were isolated and screened and the activity of filter paper enzyme was determined by feeding Hermetia illucens with waste mushroom sticks,and then using carboxymethyl cellulose sodium medium and Lugol's iodine solution staining method.The results showed that one high-yielding cellulase-decomposing bacterium was screened from the intestinal flora of Hermetia illucens,with a D/d value of 4.17 and a filter paper enzyme activity of 100.43 U/mL,indicating that it had a high cellulose degradation ability.

关 键 词：纤维素 纤维素酶 纤维素降解菌 筛选 黑水虻 

分 类 号：Q939[生物学—微生物学]