SMC1A在乳腺癌中的表达及对乳腺癌细胞凋亡的影响  

Expression of SMC1A in breast cancer and its effect on apoptosis of breast cancer cells

作　　者：丁峰 喻夏飞 朱妍慧 杨君哲 吴娴 刘晓安[1] 邹黎[3] DING Feng;YU Xiafei;ZHU Yanhui;YANG Junzhe;WU Xian;LIU Xiao’an;ZOU Li(Department of Breast Surgery,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029;Department of General Surgery,Nanjing Qixia District Hospital,Nanjing 210046;Department of Pediatrics,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院乳腺外科,江苏南京210029 [2]南京市栖霞区医院普外科,江苏南京210046 [3]南京医科大学第一附属医院儿科,江苏南京210029

出　　处：《南京医科大学学报(自然科学版)》2025年第2期245-252,共8页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(82072931);南京医科大学科技发展基金(NMUB20220098)。

摘　　要：目的:探讨染色体结构维持蛋白1A(structural maintenance of chromosome 1A,SMC1A)在乳腺癌中的表达情况,分析其与临床病理特征的相关性以及对乳腺癌细胞凋亡的影响。方法:利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库分析SMC1A m RNA在乳腺癌及正常乳腺组织中的表达差异及其与乳腺癌预后的相关性。收集南京医科大学第一附属医院2023年1—12月间48例乳腺癌患者手术切除的癌组织和癌旁组织标本,采用实时荧光定量聚合酶链反应和免疫组织化学分析SMC1A的表达,并评估其与患者临床病理指标的关系。通过下调乳腺癌细胞系MCF-7中SMC1A表达,检测其凋亡相关蛋白的变化。结果:对TCGA相关转录组测序数据分析提示,SMC1A m RNA在乳腺癌组织中的表达显著高于正常乳腺组织,并与乳腺癌的预后紧密相关。临床样本验证也表明,SMC1A的表达水平在乳腺癌组织中显著高于癌旁组织,并与TNM分期、淋巴结转移及病理分化程度相关。siRNA介导的SMC1A下调在MCF-7细胞中显著提升了Cleaved-caspase-3、Cleaved-caspase-9及Bax的表达,降低了Bcl2的表达。结论:SMC1A在乳腺癌组织中高表达,并与乳腺癌的进展及病理分化有关。初步机制研究表明,SMC1A的表达调控可能对促进乳腺癌细胞凋亡具有重要作用。Objective:To investigate the expression of structural maintenance of chromosome 1A(SMC1A)in breast cancer,analyze its correlation with clinical pathologic features,and explore its influence on apoptosis of breast cancer cells.Methods:The study first analyzed the expression differences of SMC1A mRNA in breast cancer and healthy breast tissues and their prognostic significance using The Cancer Genome Atlas(TCGA)database.It then involved collecting tumor and adjacent non⁃tumor tissue samples from 48 breast cancer patients who underwent surgical resection at the First Affiliated Hospital of Nanjing Medical University from January to December 2023.Real⁃time quantitative polymerase chain reaction(RT⁃qPCR)and immunohistochemistry(IHC)were performed to analyze SMC1A expression and evaluate its correlation with clinical pathologic traits.Additionally,by downregulating SMC1A expression in the MCF⁃7 breast cancer cell line,apoptosis⁃related protein changes were recorded.Results:Analysis of TCGA transcriptomic sequencing data revealed that SMC1A mRNA expression was significantly higher in breast cancer tissues compared to normal breast tissues,and was closely orrelated with poorer cancer prognosis.Clinical sample validation confirmed that SMC1A expression levels were significantly elevated in breast cancer tissues compared to adjacent tissues and were correlated with TNM stage,lymph node metastasis,and pathological differentiation.Downregulation of SMC1A via siRNA1 in MCF⁃7 cells notably increased Cleaved⁃caspase⁃3,Cleaved⁃caspase⁃9,and Bax levels,while decreased Bcl2 levels.Conclusion:SMC1A is highly expressed in breast cancer tissues,correlating with disease progression and pathological differentiation.Early mechanistic investigations indicate that reducing SMC1A expression may promote apoptosis in breast cancer cells,suggesting its regulatory role could be critical in mitigating breast cancer progression.

关 键 词：乳腺癌 SMC1A 细胞凋亡 癌症基因组图谱 

分 类 号：R737.9[医药卫生—肿瘤]