单细胞转录组测序技术在胃癌肿瘤微环境重编程研究中的应用进展  

Progress of single⁃cell RNA sequencing technology in the study of tumor microenvironment reprogramming in gastric cancer

作　　者：胡非凡 占强 安方梅 HU Feifan;ZHAN Qiang;AN Fangmei(Department of Gastroenterology,the Affiliated Wuxi People′s Hospital of Nanjing Medical University,Wuxi Medical Center,Nanjing Medical University,Jiangsu Branch of the National Clinical Research Center for Digestive Diseases,Wuxi 214023,China)

机构地区：[1]南京医科大学附属无锡人民医院消化内科,南京医科大学无锡医学中心,国家消化系统疾病临床医学研究中心(西安)江苏省分中心,江苏无锡214023

出　　处：《南京医科大学学报(自然科学版)》2025年第2期285-290,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金(BK20211039);无锡市双百拔尖人才项目(BJ2023008);南京医科大学无锡医学中心专病队列和临床研究项目(WMCC202302);南京医科大学无锡医学中心重点项目(WMCM202305)。

摘　　要：胃癌发病和肿瘤微环境重编程密切相关,但肿瘤微环境的复杂性与异质性限制了胃癌的个体化精准诊疗。单细胞转录组测序技术从探讨肿瘤微环境重编程过程出发,可在单细胞水平“描绘”胃癌中肿瘤微环境的“景观”,深入探索肿瘤微环境各细胞亚型间的相互作用,揭示肿瘤微环境调控胃癌进展与转移的免疫新机制,探究肿瘤微环境诱导胃癌化疗耐药的分子机制,挖掘肿瘤微环境中胃癌潜在的免疫治疗新靶点,提供预测肿瘤微环境影响胃癌预后的新方法等,为研究胃癌起源、发病机制、疾病进展以及免疫耐受等提供更全面、精准的证据。文章就单细胞转录组测序技术在胃癌微环境重编程研究中的应用现状作一综述,力求为胃癌的精准诊治提供新思路。Gastric cancer is closely related to the reprogramming of the tumor microenvironment,but the complexity and heterogeneity of the tumor microenvironment presents a significant challenge to the development of individualized and precise diagnose and treatment strategies for gastric cancer.Focusing on the reprogramming process of tumor microenvironment,single⁃cell sequencing technology can depict the“landscape”of the gastric cancer tumor microenvironment at the single⁃cell level,explore the interactions between cell subtypes and their components,reveal the novel immune mechanisms by which the tumor microenvironment regulates the progression and metastasis of gastric cancer,explore the molecular mechanism of chemo⁃resistance in the tumor microenvironment of gastric cancer,identify the potential immunotherapy targets for gastric cancer within the tumor microenvironment,and provide a new prediction method for the prognosis of gastric cancer.The technology will provide more comprehensive and precise evidence for the study of origin,pathogenesis,progression,and immune tolerance in gastric cancer.The following paper reviews the application of single⁃cell RNA sequencing technology in the reprogramming of the tumor microenvironment in gastric cancer and attempts to provide new ideas for the precise diagnosis and treatment of gastric cancer.

关 键 词：胃癌 肿瘤微环境 单细胞转录组测序 异质性 

分 类 号：R735.2[医药卫生—肿瘤]