CD62P、CD40L及节律核受体Rev-erbα在缺血性脑卒中不同发病时间的表达  

The expressions of CD62P,CD40L,and Rev-erbαafter ischemic stroke on different onset times

作　　者：王银平 孟灿灿 吴文娟 杨直堂[2] Wang Yinping;Meng Cancan;Wu Wenjuan;Yang Zhitang(Clinical Medical College of Hebei Engineering University,Handan 056000,Hebei Province,China;Department of Neurology,Affiliated Hospital of Hebei Engineering University,Handan 056000,Hebei Province,China)

机构地区：[1]河北工程大学临床医学院临床医学专业,河北邯郸056000 [2]河北工程大学附属医院神经内科,河北邯郸056000

出　　处：《首都医科大学学报》2025年第1期34-40,共7页Journal of Capital Medical University

基　　金：国家自然科学基金项目(82271507);河北省卫健委项目(20240968);邯郸市科学技术研究与发展计划项目(19422083011-5);河北省政府资助优秀人才项目。

摘　　要：目的研究缺血性脑卒中患者不同发病时间的节律核受体Rev-erbα及血小板活化因子P-选择素(CD62P)、肿瘤坏死因子CD40L表达水平,为缺血性脑卒中提供新的治疗方法。方法将158例因缺血性脑卒中住院的患者作为缺血性脑卒中组,根据发病时间分为4个亚组:T_(1)(0~6点)组32人、T_(2)(6~12点)组50人、T_(3)(12~18点)组40人、T_(4)(18~24点)组36人。将同时间非缺血性脑卒中住院的126例患者作为对照组,分别为T_(1)组32人,T_(2)组31人,T_(3)组32人,T_(4)组31人。患者入院后采集血浆和血小板标本,通过酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测CD62P、CD40L及Rev-erbα表达水平。结果缺血性脑卒中组T_(2)时间段CD62P、CD40L及Rev-erbα的表达明显高于其他时间段(P<0.05),且CD62P及CD40L表达水平变化与Rev-erbα的表达变化相一致,而T_(1)、T_(3)、T_(4)三组之间的CD62P、CD40L与Rev-erbα的表达差异无统计学意义(P>0.05)。缺血性脑卒中组各时间段CD62P、CD40L和Rev-erbα表达均明显高于对照组(P<0.05),结论节律核受体Rev-erbα及血小板活化水平在缺血性脑卒中后表达明显增高,特别是在凌晨时间段,并且血小板活化水平与节律核受体Rev-erbα表达在时间上是一致的,提示Rev-erbα与血小板活化在缺血性脑卒中发病中发挥了重要作用,并且它们之间存在明显的关联。Objective To investigate the expression levels of platelet activating factor and rhythm nuclear receptor Rev-erbαin patients after ischemic stroke on different onset times.Methods A total of 158 patients after ischemic stroke admitted from June 2022 to May 2023 were divided into four groups according to the onset time T_(1)(0-6 o'clock),T_(2)(6-12 o'clock),T_(3)(12-18 o'clock),and T_(4)(18-24 o'clock).Matched to the ischemic stroke groups,126 patients hospitalized at the same time with non-ischemic stroke were divided into four groups.General data and serum samples of the subjects were collected,and the expression levels of CD62P,CD40L and Rev-erbαwere detected with enzyme-linked immunosorbent assay(ELISA).Results The expressions of platelet activating factor CD62P,tumor necrosis factor CD40L,and rhythm nuclear receptor Rev-erbαin T_(2) group after ischemic stroke were significantly higher than that in the other groups(P<0.05),whilst no significant difference were observed in other three groups(P>0.05).The expression of CD62P,CD40L and rev-erbαin ischemic stroke groups were significantly higher than that in control groups.Conclusion The expression levels of the rhythm nuclear receptor Rev-erbα,CD62P,and CD40L in ischemic stroke groups is higher than that in control groups,and the expression of these cytokines is more significant in T_(2) group(i.e.early in the morning)than other time slot.On the other hand,the expressions of CD62P and CD40L are consistent with Rev-erbαon the time slots,indicating a clear correlation.It is indicated Rev-erbαand platelet activation play a key role on attacking of ischemic stroke.

关 键 词：缺血性脑卒中 血小板活化 CD62P CD40L 节律核受体Rev-erbα 

分 类 号：R743.3[医药卫生—神经病学与精神病学]