Multifunctional computational fluorescence self-interference holographic microscopy  

作　　者：WENXUE ZHANG TIANLONG MAN MINGHUA ZHANG HONGQIANG ZHOU ZENGHUA LIU YUHONG WAN 

机构地区：[1]School of Physics and Optoelectronic Engineering,Beijing University of Technology,Beijing 100124,China [2]School of Information Science and Technology,Beijing University of Technology,Beijing 100124,China

出　　处：《Photonics Research》2024年第11期2703-2711,共9页光子学研究(英文版)

基　　金：Beijing Municipal Natural Science Foundation(3222001,4182016);National Natural Science Foundation of China(61575009)。

摘　　要：Fluorescence microscopy is crucial in various fields such as biology,medicine,and life sciences.Fluorescence selfinterference holographic microscopy has great potential in bio-imaging owing to its unique wavefront coding characteristics;thus,it can be employed as three-dimensional(3D)scanning-free super-resolution microscopy.However,the available approaches are limited to low optical efficiency,complex optical setups,and single imaging functions.The geometric phase lens can efficiently manipulate the optical field’s amplitude,phase,and polarization.Inspired by geometric phase and self-interference holography,a self-interference fluorescent holographic microscope-based geometric phase lens is proposed.This system allows for wide-field,3D fluorescence holographic imaging,and edge-enhancement from the reconstruction of only one complex-valued hologram.Experiments demonstrate the effectiveness of our method in imaging biological samples,with improved resolution and signal-to-noise ratio.Furthermore,its simplicity and convenience make it easily compatible with existing optical microscope setups,making it a powerful tool for observing biological samples and detecting industrial defects.

关 键 词：FUNCTIONS HOLOGRAPHIC FLUORESCENCE 

分 类 号：O43[机械工程—光学工程]