VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture  

作　　者：Yan Xu Zhengxin Lv Muhammad Aamir Manzoor Linhong Song Maosen Wang Lei Wang Shiping Wang Caixi Zhang Songtao Jiu 

机构地区：[1]Department of Plant Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Dongchuan Road No.800,Shanghai 200240,P.R.China

出　　处：《Molecular Horticulture》2024年第1期94-109,共16页分子园艺(英文)

基　　金：funded by the National Natural Science Foundation of China(32102346);Shanghai Sailing Program(21YF1422100);National Postdoctoral Program for Innovative Talents(BX20180199);Startup Fund for Young Faculty at SJTU(21×010500643).

摘　　要：The D14 protein,an alpha/beta hydrolase,is a key receptor in the strigolactone(SL)signaling pathway.However,the response of VvD14 to SL signals and its role in grapevine root architecture formation remain unclear.This study demonstrated that VvD14c was highly expressed in grapevine tissues and fruit stages than other VvD14 isoforms.Application of GR24,an SL analog,enhanced the elongation and diameter of adventitious roots but inhibited the elongation and density of lateral roots(LRs)and increased VvD14c expression.Additionally,GR24 is nested within the VvD14c pocket and strongly bound to the VvD14c protein,with an affinity of 5.65×10^(-9) M.Furthermore,VvD14c interacted with grapevine MORE AXILLARY GROWTH 2(VvMAX2)in a GR24-dependent manner.Overexpression of VvD14c in the d14 mutant and VvMAX2 in the max2 Arabidopsis mutant reversed the increased LR number and density,as well as primary root elongation.Conversely,homologous overexpression of VvD14c and VvMAX2 resulted in reduced LR number and density in grapevines.VvMAX2 directly interacted with LATERAL ORGAN BOUNDARY(VvLOB)and VvLBD19,thereby positively regulating LR density.These findings highlight the role of SLs in regulating grapevine root architecture,potentially via the VvD14c-VvMAX2-VvLOB/VvLBD19 module,providing new insights into the regulation of root growth and development in grapevines.

关 键 词：GRAPEVINES STRIGOLACTONES Root architecture DWARF 14 MORE AXILLARY GROWTH 2 

分 类 号：G63[文化科学—教育学]