LcMPK3 and LcMPK6 positively regulate fruitlet abscission in litchi  

作　　者：Fei Wang Zhijian Liang Xingshuai Ma Zidi He Jianguo Li Minglei Zhao 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro‑Bioresources,South China Agricultural University,Guangzhou 510642,China [2]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China),Ministry of Agriculture and Rural Affairs,South China Agricultural University,Guangzhou 510642,China [3]Guangdong Litchi Engineering Research Center,College of Horticulture,South China Agricultural University,Guangzhou 510642,China

出　　处：《Molecular Horticulture》2024年第1期235-252,共18页分子园艺(英文)

基　　金：supported by grants from the Natural Science Foundation of Guangdong Province,China(2021B1515120082);the National Natural Science Foundation of China(32330092 and 32072544).

摘　　要：Mitogen-activated protein kinase(MAPK)cascades have been discovered to play a fundamental role in regulating organ abscission.However,the identity of protein substrates targeted by MAPK cascades,as well as whether the role of MAPK protein cascades in the abscission process is conserved across different plant species,remain unknown.Here,the role of homologs of MPK3 and MPK6 in regulating fruit abscission were characterized in litchi.Ectopic expression of LcMPK3 or LcMPK6 in Arabidopsis mpk3 mpk6 mutant rescued the deficiency in floral organ abscission,while silencing of LcMPK3 or LcMPK6 in litchi significantly decreased fruitlet abscission.Importantly,a total of 49 proteins interacting with LcMPK3 were identified through yeast two-hybrid screening,including two components of the MAPK signaling cascade,five transcription factors,and two aquaporins.Furthermore,the interaction between LcMPK3/6 with LcBZR1/2,core components in brassinosteroids signaling that suppress litchi fruitlet abscission,was confirmed using in vitro and in vivo assays.Moreover,phos-tag assays demonstrated that LcMPK3/6 could phosphorylate LcBZR1/2,with several phosphorylation residues identified.Together,our findings suggest that LcMPK3 and LcMPK6 play a positive regulatory role in fruitlet abscission in litchi,and offer crucial information for the investigation of mechanisms underlying MPK3/6-mediated organ abscission in plants.

关 键 词：LITCHI Fruit abscission MPK3/6 

分 类 号：R73[医药卫生—肿瘤]