A high-quality haplotype genome of Michelia alba DC reveals differences in methylation patterns and flower characteristics  

作　　者：Sirong Jiang Meiling Zou Chenji Zhang wanfeng Ma Chengcai Xia Zixuan Li Long Zhao Qi Liu Fen Yu Dongyi Huang Zhiqiang Xia 

机构地区：[1]Sanya Nanfan Research Institute of Hainan University,Hainan Yazhou Bay Seed Laboratory,Sanya,China [2]College of Tropical Crops,Hainan University,Haikou,China [3]China Agricultural University,Beijing,China [4]Qinghai University,Xining,China

出　　处：《Molecular Horticulture》2024年第1期318-334,共17页分子园艺(英文)

基　　金：supported by a project on Hainan Provincial Project of Science and Technology(ZDYF2022XDNY149);Developing Bioinformatics Platform in Hainan Yazhou Bay Seed Lab(B21HJ0001);Supported by the PhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City(HSPHDSRF-2023-12-007);Hainan University Startup Fund(KYQD(ZR)-20101).

摘　　要：Michelia alba DC is a highly valuable ornamental plant of the Magnoliaceae family.This evergreen tropical tree commonly grows in Southeast Asia and is adored for its delightful fragrance.Our study assembled the M.alba haplotype genome MC and MM by utilizing Nanopore ultralong reads,Pacbio Hifi long reads and parental second-generation data.Moreover,the first methylation map of Magnoliaceae was constructed based on the methylation site data obtained using Nanopore data.Metabolomic datasets were generated from the flowers of three different species to assess variations in pigment and volatile compound accumulation.Finally,transcriptome data were generated to link genomic,methylation,and morphological patterns to reveal the reasons underlying the differences between M.alba and its parental lines in petal color,flower shape,and fragrance.We found that the AP1 and AP2 genes are crucial in M.alba petal formation,while the 4CL,PAL,and C4H genes control petal color.The data generated in this study serve as a foundation for future physiological and biochemical research on M.alba,facilitate the targeted improvement of M.alba varieties,and offer a theoretical basis for molecular research on Michelia L.

关 键 词：Michelia L Haplotype genome assembly DNA methylation Allele genes FLOWER 

分 类 号：R73[医药卫生—肿瘤]