Haplotype-resolved genome assembly of the diploid Rosa chinensis provides insight into the mechanisms underlying key ornamental traits  

作　　者：Xiaoni Zhang Quanshu Wu Lan Lan Dan Peng Huilin Guan Kaiqing Luo Manzhu Bao Mohammed Bendahmane Xiaopeng Fu Zhiqiang Wu 

机构地区：[1]National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China [2]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [3]Kunpeng Institute of Modern Agriculture at Foshan,Foshan 528200,China [4]College of Science,Health,Engineering and Education,Murdoch University,90 South Street,Murdoch,WA 6150,Australia [5]Laboratoire Reproduction Et Development Des Plantes,INRA-CNRS-Lyon1-ENS,Ecole Normale Supérieure de Lyon,520074 Lyon,France

出　　处：《Molecular Horticulture》2024年第1期396-411,共16页分子园艺(英文)

基　　金：supported by funding from the National key research and development program of China(2019YFD1000403&2021YFD1200205-03);The Scientific Research Foundation for Principle Investigator,Kunpeng Institute of Modern Agriculture at Foshan(KIMA-QD2022004);the Funding of Major Scientific Research Tasks,Kunpeng Institute of Modern Agriculture at Foshan(KIMA-ZDKY2022004);this work was also supported in part by the Chinese Academy of Agricultural Sciences Elite Youth Program to Zhiqiang Wu.

摘　　要：Roses are consistently ranked at the forefront in cut flower production.Increasing demands of market and changing climate conditions have resulted in the need to further improve the diversity and quality of traits.However,frequent hybridization leads to highly heterozygous nature,including the allelic variants.Therefore,the absence of comprehensive genomic information leads to them making it challenging to molecular breeding.Here,two haplotype-resolved chromosome genomes for Rosa chinensis‘Chilong Hanzhu’(2n=14)which is high heterozygous diploid old Chinese rose are generated.An amount of genetic variation(1,605,616 SNPs,209,575 indels)is identified.13,971 allelic genes show differential expression patterns between two haplotypes.Importantly,these differences hold valuable insights into regulatory mechanisms of traits.RcMYB114b can influence cyanidin-3-glucoside accumulation and the allelic variation in its promoter leads to differences in promoter activity,which as a factor control petal color.Moreover,gene family expansion may contribute to the abundance of terpenes in floral scents.Additionally,RcANT1,RcDA1,RcAG1 and RcSVP1 genes are involved in regulation of petal number and size under heat stress treatment.This study provides a foundation for molecular breeding to improve important characteristics of roses.

关 键 词：Rosa chinensis Haplotype-resolved genome Petal colour Flower development 

分 类 号：R73[医药卫生—肿瘤]