TrichomeLess Regulator 3 is required for trichome initial and cuticle biosynthesis in Artemisia annua  

作　　者：Boran Dong Zihan Xu Xingxing Wang JinXing Li Ying Xiao Doudou Huang Zongyou Lv Wansheng Chen 

机构地区：[1]Research and Development Center of Chinese Medicine Resources and Biotechnology,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [2]Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China

出　　处：《Molecular Horticulture》2024年第1期449-467,共19页分子园艺(英文)

基　　金：funded by National Natural Science Foundation of China(32070332);Shanghai local Science and Technology Development Fund Program guided by the Central Government(YDZX20203100002948);National Key R&D Program of China(2022YFC3501700,2023YFC3504800).

摘　　要：Artemisinin is primarily synthesized and stored in the subepidermal space of the glandular trichomes of Artemi-sia annua.The augmentation of trichome density has been demonstrated to enhance artemisinin yield.However,existing literature lacks insights into the correlation between the stratum corneum and trichomes.This study aims to unravel the involvement of TrichomeLess Regulator 3(TLR3),which encodes the transcription factor,in artemisinin biosynthesis and its potential association with the stratum corneum.TLR3 was identified as a candidate gene through transcriptome analysis.The role of TLR3 in trichome development and morphology was investigated using yeast two-hybrid,pull-down analysis,and RNA electrophoresis mobility assay.Our research revealed that TLR3 nega-tively regulates trichome development.It modulates the morphology of Arabidopsis thaliana trichomes by inhibiting branching and inducing the formation of abnormal trichomes in Artemisia annua.Overexpression of the TLR3 gene disrupts the arrangement of the stratum corneum and reduces artemisinin content.Simultaneously,TLR3 possesses the capacity to regulate stratum corneum development and trichome follicle morphology by interacting with TRICHOME AND ARTEMISININ REGULATOR 1,and CycTL.Consequently,our findings underscore the pivotal role of TLR3 in the development of glandular trichomes and stratum corneum biosynthesis,thereby influencing the morphology of Artemisia annua trichomes.

关 键 词：Artemisia annua ARTEMISININ Glandular trichomes Trichome morphology Cuticle Negative regulation 

分 类 号：X53[环境科学与工程—环境工程]