Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system  

作　　者：Xiaokang Zhang Zhanquan Zhang Tong Chen Yong Chen Boqiang Li Shiping Tian 

机构地区：[1]Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Molecular Horticulture》2024年第1期468-483,共16页分子园艺(英文)

基　　金：supported by the National Natural Science Foundation of China(31930086,U23A20264,31972475);the National Key R&D Program of China(2021YFD2100505).

摘　　要：Botrytis cinerea is one of the most destructive phytopathogenic fungi,causing significant losses to horticultural crops.As a necrotrophic fungus,B.cinerea obtains nutrients by killing host cells.Secreted cell death-inducing proteins(CDIPs)play a crucial role in necrotrophic infection;however,only a limited number have been reported.For highthroughput CDIP screening,we optimized the prokaryotic expression system and compared its efficiency with other commonly used protein expression systems.The optimized prokaryotic expression system showed superior effectiveness and efficiency and was selected for subsequent CDIP screening.The screening system verified fifty-five candidate proteins and identified two novel SGNH family CDIPs:BcRAE and BcFAT.BcRAE and BcFAT exhibited high expres-sion levels throughout the infection process.Site-directed mutagenesis targeting conserved Ser residues abolished the cell death-inducing activity of both BcRAE and BcFAT.Moreover,the transient expression of BcRAE and BcFAT in plants enhanced plant resistance against B.cinerea without inducing cell death,independent of their enzymatic activities.Our results suggest a high-efficiency screening system for high-throughput CDIP screening and provide new targets for further study of B.cinerea-plant interactions.

关 键 词：Botrytis cinerea Cell death-inducing protein SGNH family hydrolases Plant-fungus interaction 

分 类 号：R73[医药卫生—肿瘤]