Haplotype-resolved genome assembly provides insights into evolutionary history of the Actinidia arguta tetraploid  

作　　者：Feng Zhang Yingzhen Wang Yunzhi Lin Hongtao Wang Ying Wu Wangmei Ren Lihuan Wang Ying Yang Pengpeng Zheng Songhu Wang Junyang Yue Yongsheng Liu 

机构地区：[1]School of Horticulture,Anhui Agricultural University,Hefei 230036,China [2]School of Forestry Science and Technology,Lishui Vocational and Technical College,Lishui 323000,China [3]Ministry of Education Key Laboratory for Bio‑Resource and Eco‑Environment,College of Life Science,State Key Laboratory of Hydraulics and Mountain River Engineering,Sichuan University,Chengdu 610064,China

出　　处：《Molecular Horticulture》2024年第1期525-542,共18页分子园艺(英文)

基　　金：supported by funds from the National Natural Science Foundation of China(Grant Nos.U23A20204,31972474 and 90717110).

摘　　要：Actinidia arguta,known as hardy kiwifruit,is a widely cultivated species with distinct botanical characteristics such as small and smooth-fruited,rich in beneficial nutrients,rapid softening and tolerant to extremely low temperatures.It contains the most diverse ploidy types,including diploid,tetraploid,hexaploid,octoploid,and decaploid.Here we report a haplotype-resolved tetraploid genome(A.arguta cv.‘Longcheng No.2’)containing four haplo-types,each with 40,859,41,377,39,833 and 39,222 protein-coding genes.We described the phased genome structure,synteny,and evolutionary analyses to identify and date possible WGD events.K_(s) calculations for both allelic and paralogous genes pairs throughout the assembled haplotypic individuals showed its tetraploidization is estimated to have formed~1.03 Mya following Ad-αevent occurred~18.7 Mya.Detailed annotations of NBS-LRRs or CBFs highlight the importance of genetic variations coming about after polyploidization in underpinning ability of immune responses or environmental adaptability.WGCNA analysis of postharvest quality indicators in combination with transcriptome revealed several transcription factors were involved in regulating ripening kiwi berry texture.Taking together,the assembly of an A.arguta tetraploid genome provides valuable resources in deciphering complex genome structure and facilitating functional genomics studies and genetic improvement for kiwifruit and other crops.

关 键 词：Actinidia arguta GENOMICS POLYPLOID HAPLOTYPE Stress tolerance 

分 类 号：S66[农业科学—果树学]