采用不同溶剂提取的金耳粗多糖性质及对细胞迁移的影响  

Physicochemical Properties of Naematelia aurantialba Crude Polysaccharides Extracted with Different Solvents and Their Effects on Cell Migration

作　　者：孙浩雯 刘艳芳[2] 冯杰[2] 刘利平 杨林雷 曹瑶 张劲松[2] 康继 SUN Haowen;LIU Yanfang;FENG Jie;LIU Liping;YANG Linlei;CAO Yao;ZHANG Jingsong;KANG Ji(College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;Institute of Edible Fungi Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Yunnan Junshijie Biotechnology Co.,Ltd.,Kunming 651708,Yunnan,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403 [3]云南菌视界生物科技有限公司,云南昆明651708

出　　处：《食用菌学报》2025年第1期53-64,共12页Acta Edulis Fungi

基　　金：云南(昆明)张劲松药用真菌专家工作站(YSZJGZZ-2022043);云南省张劲松专家工作站(202305AF150084)。

摘　　要：为探讨采用不同溶剂提取的金耳(Naematelia aurantialba)粗多糖的化学成分、结构特征、流变特性及其对细胞迁移的影响,分别以蒸馏水、0.1 mol·L^(-1)NaOH溶液、0.1mol·L^(-1)HCl溶液为溶剂提取子实体的粗多糖,采用苯酚-硫酸法、二硝基水杨酸法和间羟基联苯法分别测定总糖、还原糖和糖醛酸的含量,采用高效凝胶尺寸排阻色谱-多角度激光光散射仪-示差折光检测仪(high performance size exclusion chromatography-multiangle laser light scattering-refractive index,HPSEC-MALLS-RI)联用系统测定分子量,采用傅里叶变换红外光谱仪检测多糖的特征基团,采用离子色谱仪测定单糖组成,采用流变仪测定表观黏度和模量,通过细胞实验测定细胞毒性和细胞迁移率。结果表明:水提取金耳粗多糖(NAP-WE)的得率较高,碱提取金耳粗多糖(NAP-AlE)的多糖和糖醛酸含量较高,而酸提取金耳粗多糖(NAP-AcE)的蛋白质含量较高;NAP-WE重均分子量较大,为2.462×10^(6)g·mol^(-1),多分散系数为1.515;NAP-AlE重均分子量为1.187×10^(6)g·mol^(-1),多分散系数为1.530;NAP-AcE重均分子量为4.545×10^(5)g·mol^(-1),比NAP-WE和NAPAlE的低,多分散系数为2.501;3种粗多糖均具有多糖的羟基、羧基、α和β糖苷键特征吸收峰,均由木糖、甘露糖、葡萄糖醛酸以及少量葡萄糖组成,NAP-AlE的葡萄糖摩尔百分比和葡萄糖醛酸摩尔百分比较高,但木糖摩尔百分比较低;NAP-AlE和NAP-AcE的甘露糖摩尔百分比较高,而NAP-WE的较低;当质量浓度为40~60mg·mL^(-1)时,表观黏度NAP-WE>NAP-AlE>NAP-AcE;当质量浓度为30~60 mg·mL^(-1)时,NAP-WE能够形成缠结网络结构;当质量浓度为60 mg·mL^(-1)时,NAP-AlE能够形成缠结网络结构;当质量浓度为20~60 mg·mL^(-1)时,NAP-AcE为流体状态;当质量浓度为50~800μg·mL^(-1)时,3种粗多糖对细胞无明显毒性;当质量浓度为200~800μg·mL^(-1)时,3种粗多糖均可促进细胞迁移。该结果可为金耳多�The effects of different extraction solvents on the chemical composition,structural characteristics,and rheological properties of Naematelia aurantialba crude polysaccharide extracts were studied.The crude polysacchride extracts were also studied for their toxicity to HaCaT cells and effect on HaCaT migration.Different solvents,distilled water,0.1 mol·L^(-1) NaOH solution and 0.1 mol·L^(-1) HCl solution,were used to extract fruiting bodies of N.aurantialba to generate three crude polysaccharide extracts NAP-WE(extracted by water),NAP-AlE(extracted by NaOH)and NAP-AcE(extracted by HCl),respectively.NAP-WE,NAP-AlE and NAP-AcE were then determined for total sugar content by the phenol-sulfuric acid method,reducing sugar content by the dinitrosalicylic acid method,uronic acid content by the m—hydroxy-biphenyl method,molecular weight and distribution by high performance size exclusion chromatography-multiangle laser light scatteringrefractive index(HPSEC-MALLS-RI),characteristic groups by Fourier transform infrared spectrometry,monosaccharide composition by ion chromatography,apparent viscosity and modulus by a rheometer,and cytotoxicity and cell mobility in vitro.The results showed that the yield of NAP-WE was higher than the other two crude polysaccharide extracts,the contents of polysaccharide and uronic acid in NAP-AlE were higher than those in the other two crude polysaccharide extracts,and the protein content of NAP-AcE was higher than that of the other two crude polysaccharide extracts.NAP-WE had a large Mw of 2.462×10^(6) g·mol^(-1),and its polydispersion coefficient(Mw/Mn)was 1.515.The Mw and polydispersion coefficient of NAP-AlE were 1.187×10^(6) g·mol^(-1) and 1.530,respectively.NAP-AcE had the lowest Mw of 4.545×10^(5) g·mol^(-1) among the three crude polysaccharide extracts,and its polydispersion coefficient was 2.501.All three kinds of crude polysaccharide extracts contained the characteristic polysaccharide absorption peaks of hydroxyl,carboxyl,αandβglucosidic bonds,and were composed of xylo

关 键 词：金耳 多糖 性质 细胞迁移 

分 类 号：S646[农业科学—蔬菜学]