一个新的小麦黄叶位点的分子定位  

Molecular Mapping of a Novel Yellow Leaf Locus in Wheat

作　　者：闫贵云 左静静 王敏[1] 罗仕银 谭丹 乔麟轶 YAN Guiyun;ZUO Jingjing;WANG Min;LUO Shiyin;TAN Dan;QIAO Linyi(College of Agriculture,Shanxi Agricultural University/Shanxi Key Laboratory of Crop Genetics and Molecular Improvement,Taiyuan 030031)

机构地区：[1]山西农业大学农学院/作物遗传与分子改良山西省重点实验室,太原030031

出　　处：《植物遗传资源学报》2025年第1期157-164,共8页Journal of Plant Genetic Resources

基　　金：中央引导地方科技发展资金项目(YDZJSX20231A037);山西省基础研究计划青年科学研究项目(202203021212418)。

摘　　要：小麦叶片颜色与植株光合效率和籽粒产量密切相关。人工合成小麦Y223表现为生理性黄叶,与正常绿叶品种晋作82的杂种F_1植株叶色表现为中间型,在Y223旗叶抽出17 d后,杂种F_2群体中绿叶、中间和黄叶类型植株数目的分离比例符合1∶2∶1,表明Y223的黄叶表现不完全显性。采用混合分离群体分析法,利用杂种F_2植株构建绿叶池和黄叶池,从分布于小麦21条染色体的569个SSR标记中筛选出12个在亲本间和绿叶/黄叶池间表现多态性的标记。利用9个位于2B染色体的多态性标记扩增F_2群体,最终用5个标记定位了黄叶位点,暂命名为YL-2B,其侧翼标记为Xbarc200和Xbarc55,与YL-2B的遗传距离分别为2.3 cM和3.8 cM。通过比较显隐性、黄叶表型和标记连锁程度,证明YL-2B与2B染色体上已报道黄叶位点均不相同,是1个新的黄叶位点。本研究初步明确了Y223黄叶位点的遗传规律和染色体位置,为下一步基因克隆以及叶片衰老机制解析奠定了基础。Leaf color is closely related to plant photosynthetic efficiency and grain yield of wheat.The physiological yellow leaves were present in an artificially synthesized wheat Y223.The F1 plants derived from the cross between Y223 and a normal green leaf variety Jinzuo 82 displayed intermediate phenotype in leaf color.Seventeen days after the emergence of Y223’s flag leaves,the segregation ratio for green leaf,intermediate,and yellow leaf types in the F2 population Y223×Jinzuo 82 fits to 1∶2∶1,showing incomplete dominance of Y223’s yellow leaves.Using the bulked segregant analysis method,F2 individuals with contrasting phenotypes were selected to establish green leaf pools and yellow leaf pools.From 569 SSR markers distributed on 21 chromosomes of wheat,12 markers with consistent polymorphism between parents and green/yellow leaf pools were identified.Nine of them were located on chromosome 2B.These markers were used to genotype the F2 population,and the yellow leaf locus,designated YL-2B,was mapped by five markers.YL-2B was flanked by markers Xbarc200 and Xbarc55,with genetic distances of 2.3 cM and 3.8 cM,respectively.By comparing the characteristic of dominance/recessiveness,yellow leaf phenotype,and marker linkage,YL-2B proved to differ from the reported yellow leaf loci on chromosome 2B,indicating that it is possibly a novel locus.This study clarifies the inheritance mode and chromosome position of the yellow leaf locus carried by Y223,which provides a reference information for gene cloning and understanding leaf senescence mechanism.

关 键 词：人工合成小麦 黄叶 混合分离群体分析 SSR标记 分子定位 

分 类 号：S512.1[农业科学—作物学]