LncRNA-H19通过miR-29a-3pFzd4信号轴影响急性B细胞白血病进展的研究  

LncRNA-H19 regulates the progression of B-cell acute lymphoblastic leukemia through the miR-29a-3p/Fzd4 axis

作　　者：沈文翠[1] 刘畅 张红莉[1] 石雨薇[1] SHEN Wencui;LIU Chang;ZHANG Hongli(Department of Hematology,the Second Affiliated Hospital of Xinjiang Medical University,Xinjiang,Urumqi 830000,China;不详)

机构地区：[1]新疆医科大学第二附属医院血液科,乌鲁木齐市830000 [2]新疆维吾尔自治区乌鲁木齐市水磨沟区疾病预防控制中心传染病、地方病防制科

出　　处：《河北医药》2025年第1期5-10,共6页Hebei Medical Journal

基　　金：新疆维吾尔自治区自然科学基金资助项目(编号:2022D01C519)。

摘　　要：目的 检测LncRNA-H19对急性B细胞白血病细胞的影响,探讨LncRNA-H19发挥功能的具体机制。方法 首先在GEPIA和GEO数据库中检索LncRNA-H19和miR-29a-3p在急性B细胞白血病中的表达情况,通过qPCR在NALM-6和BALL-1细胞系中验证这些结果。通过转染技术敲低LncRNA-H19或过表达miR-29a-3p,利用CCK8和流式细胞术检测细胞增殖和凋亡情况。结果 急性B细胞白血病中,LncRNA-H19的表达显著升高(P<0.05),而miR-29a-3p的表达水平显著下降(P<0.05)。敲低LncRNA-H19或过表达miR-29a-3p均可以抑制NALM-6和BALL-1的增殖(P<0.05),并增加其凋亡(P<0.01)。此外,miR-29a-3p可以与Fzd4结合,二者在数据库中的表达呈负相关。进一步研究还发现LncRNA-H19通过miR-29a-3p/Fzd4信号轴影响Wnt/β-Catenin信号的通路。结论 LncRNA-H19在急性B细胞白血病中的高表达,以及其对白血病细胞生长和凋亡的影响;同时,还发现LncRNA-H19通过miR-29a-3p/Fzd4信号轴影响Wnt/β-Catenin信号的通路;这些发现为进一步研究LncRNA-H19在急性B细胞白血病中的作用机制以及开发相关的治疗策略提供了新的思路。Objective This study aims to detect the effect of long non-coding RNA H19(lncRNA-H19)on B-cell acute lymphoblastic leukemia(B-ALL)and the underlying mechanism.Methods Expression levels of lncRNA-H19 and miR-29a-3p in B-ALL samples were analyzed using Gene Expression Profiling Interactive Analysis(GEPIA)and Gene Expression Omnibus(GEO)databases,which were verified in NALM-6 and BALL-1 cells by the quantitative polymerase chain reaction(qPCR).After knockdown of lncRNA-H19 or overexpression of miR-29a-3p by cell transfection,proliferation and apoptosis were evaluated by CCK-8 assay and flow cytometry,respectively.Results LncRNA-H19 was significantly upregulated(P<0.05),while miR-29a-3p was significantly downregulated in B-ALL samples(P<0.05).Knockdown of lncRNA-H19 or overexpression of miR-29a-3p inhibited the proliferation(P<0.05)and induced apoptosis of NALM-6 and BALL-1 cells(P<0.01).MiR-29a-3p was found to directly target Fzd4,showing a negative correlation in B-ALL samples from online databases.Furthermore,lncRNA-H19 was found to regulate the Wnt/β-catenin pathway through the miR-29a-3p/Fzd4 axis.Conclusion LncRNA-H19 is upregulated in B-ALL cases and mediates the growth and apoptosis of B-ALL cells.It regulates the Wnt/β-catenin pathway through the miR-29a-3p/Fzd4 axis.Our findings provide new insights into the role of lncRNA-H19 in the pathogenesis of B-ALL and therapeutic strategies.

关 键 词：B细胞白血病 LncRNA-H19 miR-29a-3p Fzd4 

分 类 号：R733.71[医药卫生—肿瘤]