基于载体构建的本质理解结合软件模拟优化分子克隆教学模式  

Optimizing the teaching mode of molecular cloning based on the essential understanding of vector construction combined with the simulation software

作　　者：胡彬彬[1] 赵珂嘉 Hu Bin-bin;Zhao Ke-jia(West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西医院,四川成都610041

出　　处：《四川生理科学杂志》2025年第1期229-232,共4页

摘　　要：分子克隆技术是诸多研究领域中的关键技术,然而其复杂的操作流程、众多的技术要点以及生硬的专业名词使得学生难以快速入门。传统的教学模式往往着重于理论的讲解,缺乏系统性地归纳总结以及与实践相结合的直观展示。因而,在深入理解分子克隆的本质上,本文创新性地将其与Snapgene软件模拟相结合,指出各类载体构建的本质是通过某种手段使插入片段与载体之间形成互补配对的粘性末端。我们进一步以双酶切策略及同源重组策略为例,结合Snapgene软件演示了多个关键步骤,通过直观的图形展示以达到提高学生理解与实践能力的目的。该教学模式有望在分子生物学课程中起示范作用并推动教学方法创新。Molecular cloning technology is a key technology in many research fields,but its complex operation process,numerous technical points and rigid terminology make it difficult for students to quickly get started.The traditional teaching mode often focuses on the explanation of theory,and lacks systematic summary and intuitive display combined with practice.Therefore,in order to deeply understand the nature of molecular cloning,this paper innovatively combined it with Snapgene software simulation,pointing out that the essence of various vector construction is to form a complementary pair of sticky ends between the inserted fragment and the vector by some means.We further took the double enzyme digestion strategy and homologous recombination strategy as examples,combined with Snapgene software to demonstrate several key steps,and achieved the purpose of improving students'understanding and practical ability through intuitive graphical display.This teaching model is expected to play an exemplary role in molecular biology courses and promote the innovation of teaching methods.

关 键 词：分子克隆 载体构建 教学模式 模拟软件 

分 类 号：G642[文化科学—高等教育学]