黑灵芝多糖改善短肠综合征大鼠肠屏障功能并激活Wnt/β-catenin信号通路  

Polysaccharide from Ganoderma lucidum improves intestinal barrier function and activates Wnt/β-catenin signal pathway in rats with short bowel syndrome

作　　者：董晓霞 李云[2] 常健[2] 刘琳 郭淑芳[2] 于进洪[2] 董冰[2] DONG Xiaoxia;LI Yun;CHANG Jian;LIU Lin;GUO Shufang;YU Jinhong;DONG Bing(Nutrition Department,Handan Central Hospital,Handan 056000,China;Gastroenterology Department,Handan Central Hospital,Handan 056000,China)

机构地区：[1]邯郸市中心医院营养科,056000 [2]邯郸市中心医院消化内科,056000

出　　处：《免疫学杂志》2024年第9期727-731,共5页Immunological Journal

基　　金：邯郸市科学技术研究与发展计划(19422083009-27)。

摘　　要：目的探究黑灵芝多糖(polysaccharide from Ganoderma lucidum,PSG-1)对短肠综合征(short bowel syndrome,SBS)大鼠肠屏障功能及Wnt/β-catenin信号通路的影响。方法SD雄性大鼠50只随机分为假手术组、PSG-1低剂量组(25 mg·kg^(-1)·d^(-1))、PSG-1中剂量组(50 mg·kg^(-1)·d^(-1))、PSG-1高剂量组(100 mg/·kg^(-1)·d^(-1))。构建SBS大鼠模型,检测大鼠肠通透性,HE染色观察肠组织病理学,计算细菌移位率检测,ELISA法检测回肠组织炎性细胞因子白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子α(TNF-α)的含量,免疫荧光法检测回肠紧密连接蛋白的表达量及分布,Western blot检测回肠组织Wnt、β-catenin蛋白表达。结果与对照组相比,SBS组大鼠回肠组织绒毛较为稀疏,绒毛长度、隐窝深度、异硫氰酸荧光素-葡聚糖(FD-40)浓度、细菌移位率(肠系膜淋巴结、肝脏、脾脏)、IL-6、TNF-α水平显著升高,IL-10、Wnt、β-catenin、claudin-1、occludin荧光密度和蛋白水平显著降低;与SBS组大鼠相比,PSG-1低、中、高剂量组FD-40浓度、细菌移位率(肠系膜淋巴结、肝脏、脾脏)、IL-6、TNF-α水平显著降低,绒毛长度、隐窝深度、IL-10、Wnt、β-catenin、claudin-1、occludin荧光密度和蛋白水平显著升高。结论PSG-1可能通过激活SBS大鼠Wnt/β-catenin通路蛋白表达,减少炎症反应和肠道通透性,改善肠屏障功能障碍。Objective To investigate the impacts of polysaccharide from Ganoderma lucidum(PSG-1)on intestinal barrier function and Wnt/β-catenin signal pathway in rats with short bowel syndrome(SBS).Methods Fifty male SD rats were randomly divided into sham operation group,low dose PSG-1 group(25 mg∙kg^(-1)·d^(-1)),medium dose PSG-1 group(50 mg∙kg^(-1)·d^(-1))and high dose PSG-1 group(100 mg∙kg^(-1)·d^(-1)).SBS rat model was built,intestinal permeability of rats was detected,intestinal histopathology was observed with HE staining,and the bacterial translocation rate was calculated,the contents of inflammatory cytokines such as interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)were measured by ELISA,immunofluorescence was used to detect the expression and distribution of tight junction protein in ileum,Western blot was used to detect the expression of Wnt andβ-catenin.Results Compared with the control group,the villi of ileum in SBS group were sparse,and the villi length,recess depth,Fluorescein isothiocyanate-dextran(FD-40)concentration,bacterial translocation rate(MLN,liver,spleen),levels of IL-6 and TNF-αwere significantly increased,the levels of IL-10,Wnt,β-catenin,and the fluorescence densities and protein levels of claudin-1 and occludin were significantly decreased;compared with rats in SBS group,the FD-40 concentration,bacterial translocation rate(MLN,liver,spleen),levels of IL-6 and TNF-αin PSG-1 low,middle and high dose groups were significantly reduced,the villus length,recess depth,the levels of IL-10,Wnt,β-catenin,and the fluorescence densities and protein levels of claudin-1 and occludin were significantly increased.Conclusion PSG-1 may reduce inflammatory reaction and intestinal permeability and improve intestinal barrier dysfunction by activating the expression of Wnt/β-catenin pathway protein in SBS rats.

关 键 词：短肠综合征 肠屏障功能 黑灵芝多糖 WNT/Β-CATENIN 

分 类 号：R574.5[医药卫生—消化系统]