紫山药干腐病的病原鉴定  

Pathogen identification of dry rot of purple yam

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作  者:徐元柳 黄磊 刘敏 杨家伟 白庆荣 杨超 XU Yuanliu;HUANG Lei;LIU Min;YANG Jiawei;BAI Qingrong;YANG Chao(State Key Laboratory of Green Pesticides,Guizhou University,Guiyang 550025,Guizhou,China;Xingyi Agricultural Technology Extension Center,Xingyi 562400,Guizhou,China;Guizhou Jifeng Seed Industry Co.,Ltd,Xingyi 562400,Guizhou,China)

机构地区:[1]贵州大学绿色农药全国重点实验室,贵阳550025 [2]兴义市农业技术推广中心,贵州兴义562400 [3]贵州吉丰种业有限责任公司,贵州兴义562400

出  处:《中国瓜菜》2025年第2期129-133,共5页China Cucurbits And Vegetables

基  金:国家重点研发计划(2023YFE0104800);贵州省科技计划(黔科合支撑﹝2023﹞一般023)。

摘  要:为明确2023年在贵州省黔西南州兴义市引起当地特色作物紫山药干腐病的病原菌的分类地位,采用组织分离法和单孢纯化法获得培养特征一致的15个菌株。代表菌株SY2-2经柯氏验证为病原物,结合形态学特征观察及ITS与RPB2基因序列分析,构建系统进化树,确定引起紫山药干腐病的病原菌为产核青霉菌Penicillium sclerotigenum。该研究结果为贵州省紫山药干腐病的科学防控奠定了理论基础。To clarify the taxonomic status of the pathogen causing dry rot of purple yam in Xingyi city,Guizhou province in 2023,15 strains with the same cultural characteristics were obtained by tissue isolation and single spore purification.The representative strain SY2-2 was confirmed as pathogen of the disease by Koch's test.Based on morphological charac-teristics and ITS and RPB2 sequence analysis,a phylogenetic tree was constructed.Penicillium sclerotigenum was identi-fied as the pathogen causing the dry rot of purple yam.The results lay a theoretical foundation for the scientific control of dry rot of purple yam in Guizhou province.

关 键 词:紫山药 干腐病 病原鉴定 

分 类 号:S632.1[农业科学—蔬菜学]

 

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