花叶矢竹叶片色素合成和光合特性  

Pigment synthesis and photosynthetic characteristics of leaves in Pseudosasa japonica f.akebonosuji

作　　者：王妮 李朝娜[1] 郑旭理 姜思成 杨海芸[1] WANG Ni;LI Zhao-Na;ZHENG Xu-Li;JIANG Si-Cheng;YANG Hai-Yun(Bamboo Research Institute,Key Laboratory of Bamboo Science and Technology,Ministry of Education,State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China;Anji County Lingfeng Temple Forest Farm,Anji,Zhejiang 313300,China)

机构地区：[1]浙江农林大学,省部共建亚热带森林培育国家重点实验室,竹业科学与技术教育部重点实验室,竹子研究院,杭州311300 [2]安吉县灵峰寺林场,浙江安吉313300

出　　处：《植物生态学报》2024年第11期1536-1546,共11页Chinese Journal of Plant Ecology

基　　金：国家自然科学基金(31901370)。

摘　　要：竹子在自然界中存在较多叶色变异,花叶矢竹(Pseudosasa japonica f.akebonosuji)叶色变异是典型代表,通过对花叶矢竹不同叶色叶片光合特性的研究,可解析其叶色变异机理。该研究以花叶矢竹不同叶色叶片为研究对象,利用紫外分光光度计、高效液相色谱和连续激发式荧光仪等测定不同叶色叶片的光合色素含量、叶绿素合成前体物质相对含量、光系统活性及光合效应差异,阐明花叶矢竹叶色变异的生理机制。结果表明:(1)花叶矢竹全绿型和花叶型叶片光合色素含量差异显著,花叶型叶片的叶绿素a/b值显著低于全绿型,而类胡萝卜素/叶绿素值显著高于绿叶。(2)花叶矢竹白叶和条纹白叶的叶绿素生物合成前体物质粪卟啉Ⅲ含量显著高于绿叶,而相邻产物原卟啉Ⅸ含量急剧下降,导致叶绿素a、叶绿素b含量显著降低。(3)条纹叶的净光合速率、表观量子效率等均显著低于绿叶,全白叶无光合效应。(4)以全绿叶作为对照,花叶捕获的激子将电子传递到电子传递链中初级醌受体(QA)下游的电子受体的概率(Ψ_(o))和以吸收光能为基础的性能指数(PI_(ABS))均显著降低,且PI_(ABS)的降低幅度大于Ψ_(o),白叶复绿后也没有完全恢复,表明花叶光系统Ⅱ(PSⅡ)供体侧供应电子的能力和受体侧接收电子的能力都降低,PSⅡ整体性能低于绿叶。白叶和条纹叶叶绿素K相荧光强度(F_(k))占F_(0)-F_(j)振幅的比例(W_(k))变化值显著高于绿叶(F_(0)为初始荧光强度,F_(j)为J相荧光强度),白叶复绿后其性能基本恢复。绿叶叶绿素F_(j)占F_(0)-F_(p)振幅的比例(V_(j))变化值显著低于白叶和复绿叶,与条纹绿叶无显著差异,表明PSⅡ受体侧性能在绿叶和条纹绿叶上表现一致,但是复绿叶也没有完全恢复至绿叶水平。(5)花叶和复绿叶820 nm处的光吸收量均小于绿叶,白叶复绿过程中,光系统Ⅰ(PSⅠ)最大氧化还原能力(ΔI/I_(o))值显著Aims Variation in leaf colour is natural in bamboo,and the leaf colour variation of Pseudosasa japonicaf.akebonosuji is a typical representative.The mechanism of colour variation can be resolved by studying the photosynthetic properties of different leaf colours.Methods The photosynthetic pigment content,the relative content of chlorophyll(Chl)synthesis precursors,photosystem activities and photosynthetic effect differences of leaves with different leaf colours were determined by ultraviolet spectrophotometer,high-performance liquid chromatography and continuous excitation fluorescence to elucidate the physiological mechanism of leaf colour variation of P.japonicaf.akebonosuji.Important findings(1)There were significant differences in photosynthetic pigment content between the all-green and mosaic leaves of P.japonica f.akebonosuji.The mosaic leaves’s chl a/b value was significantly lower than the all-green leaves,while the carotenoid/Chl a+b value was substantially higher than all-green leaves.(2)The chlorophyll biosynthetic precursor substance,Coprogen III,was significantly higher in albino and striped albino leaves than in green leaves.At the same time,the content of protoporphyrin IX decreased sharply,resulting in significant reductions in Chl a and Chl b content.(3)The net photosynthetic rate and apparent quantum yield of striped leaves were significantly lower than in green leaves,and there was no photosynthetic effect in all-albino leaves.(4)Using all-green leaves as a control,the overall performance of photosystem II in mosaic leaves was lower than that of green leaves.The proportion of fluorescence intensity at J-step(F_(j))in F_(0)-F_(p)(F_(0),minimal fluorescence intensity;F_(p),fluorescence intencity at P-step)amplitude of chlorophyll value of green leaves was significantly lower than that of albino and re-greened leaves.There was no significant difference with striped green leaves,indicating that PSII receptor side performance was consistent on green and striped green leaves,but re-greened leaves d

关 键 词：花叶矢竹 叶色变异 光合色素 光合系统 光合特性 

分 类 号：G63[文化科学—教育学]