香菇多糖和灵芝孢子粉对小鼠酒精性肠道损伤的保护作用  

Protective Effects of Lentinan and Ganoderma lingzhi Spore Powder on Alcoholic Intestinal Injury in Mice

作　　者：冯林海 段庆虎 王震[3] 龚凤萍 张应香 竹玮 吴瑞可 胡鑫钰 吕亚丽 FENG Linhai;DUAN Qinghu;WANG Zhen;GONG Fengping;ZHANG Yingxiang;ZHU Wei;WU Ruike;HU Xinyu;LÜYali(Fuzhai Township Comprehensive Administrative Law Enforcement Team of Zhengyang County,Zhumadian 463000,China;Xinyang Academy of Agricultural Sciences,Xinyang 464000,China;Huanghuai University,Zhumadian 463000,China;Wangwuqiao Town Comprehensive Administrative Law Enforcement Team of Zhengyang County,Zhumadian 463000,China)

机构地区：[1]正阳县傅寨乡综合行政执法大队,河南驻马店463000 [2]信阳市农业科学院,河南信阳464000 [3]黄淮学院,河南驻马店463000 [4]正阳县王勿桥乡综合行政执法大队,河南驻马店463000

出　　处：《食药用菌》2025年第1期34-36,37,38,39,共6页Edible and Medicinal Mushrooms

基　　金：河南省食用菌产业技术体系驻马店综合试验站(HARS-22-08-Z5);驻马店市重点研发专项(ZMD52DZX2023010)。

摘　　要：为探究香菇多糖和灵芝孢子粉对小鼠酒精性肠道损伤的保护作用及作用机制,通过对连续42 d进行灌胃酒精的肠道损伤小鼠开展香菇多糖、灵芝孢子粉灌胃效果的对比试验,观察不同处理组小鼠的小肠形态,测定小肠绒毛长度、隐窝深度及其比值(V/C值),检测小鼠肠道中α-淀粉酶和脂肪酶的活性以及肠道和胃中促炎因子白介素-6(IL-6)基因的表达量。结果显示,香菇多糖、灵芝孢子粉组较对照组,小鼠肠道绒毛长度分别增加93.87%和46.38%,V/C值分别提高86.79%和41.53%,α-淀粉酶活性分别提高36.45%和49.86%,脂肪酶活性分别提高28.32%和4.45%;IL-6基因平均表达量在小鼠肠道和胃部,香菇多糖组分别较对照组降低28.00%和44.00%;灵芝孢子粉组分别较对照组降低44.00%和26.00%。表明香菇多糖、灵芝孢子粉均可通过增加小鼠肠道绒毛长度和绒毛长度与隐窝深度的比值,提高小鼠肠道消化酶活性,缓解肠道炎症反应,降低酒精对小鼠肠道屏障的损伤。To explore the protective effects and mechanisms of lentinan and Ganoderma lingzhi spore powder on alcohol-induced intestinal injury in mice,a comparative study was conducted on the effects of lentinan and G.lingzhi spore powder on intestinal injury in mice treated with alcohol for 42 consecutive days.The small intestinal morphology of mice was observed in different treatment groups,the length of intestinal villi,crypt depth,and their ratio(V/C ratio)were measured,and the activity ofα-amylase and lipase in the intestinal tract of mice and the expression of pro-inflammatory factor interleukin-6(IL-6)gene in the intestine and stomach were detected.The results indicated that,compared with the control group,the lengths of intestinal villi in the lentinan(LNT)and G.lingzhi spore powder(GLSP)groups increased by 93.87%and 46.38%,respectively.The V/C ratios improved by 86.79%and 41.53%,respectively.Additionally,theα-amylase activities increased by 36.45%and 49.86%,while the lipase activities rose by 28.32%and 4.45%,respectively.The average expression levels of the IL-6 gene in the intestines and stomachs of mice in the LNT group decreased by 28.00%and 44.00%,respectively,compared with the control group.In the GLSP group,these decreases were 44.00%and 26.00%,respectively.These findings indicated that both LNT and GLSP could enhance the lengths of intestinal villi and the V/C ratios,increase the activities of intestinal digestive enzymes,alleviate intestinal inflammatory responses,and reduce alcohol-induced damage to the intestinal barrier in mice.

关 键 词：香菇多糖 灵芝孢子粉 酒精性损伤 肠道屏障 

分 类 号：S646[农业科学—蔬菜学]