北京雨燕心脏、肺脏、肌肉和血液转录组比较分析  

Comparative Analysis of Transcriptome in Heart,Lung,Muscle,and Blood of Beijing Swift

作　　者：马春湘 张昌盛 刘燕[3] 田恒玖[4] 洪锦旋 董玉兰[1] MA Chunxiang;ZHANG Changsheng;LIU Yan;TIAN Hengjiu;HONG Jinxuan;DONG Yulan(College of Veterinary Medicine,China Agricultural University,Beijing,100193,China;National Natural History Museum of China,Beijing,100050,China;Beijing Zoo Management Office,Beijing Key Laboratory of Captive Wildlife Technology,Beijing,100044,China;Beijing Wildlife Rescue Center,Beijing,101300,China)

机构地区：[1]中国农业大学动物医学院,北京100193 [2]国家自然博物馆,北京100050 [3]北京动物园管理处,圈养野生动物技术北京市重点实验室,北京100044 [4]北京市野生动物救护中心,北京101300

出　　处：《野生动物学报》2025年第1期143-152,共10页CHINESE JOURNAL OF WILDLIFE

基　　金：北京市政府资助项目。

摘　　要：为进一步丰富北京雨燕(Apus apus pekinensis)基因组数据,采用Illumina NovaSeq6000测序平台对其肌肉、肺脏、心脏及血液转录组进行测序。结果显示:经质控后得到32.80 Gb的clean data数据,通过Trinity组装后,运用生物信息学方法与相关数据库比对,得到15295个(71.3%)有功能注释的unigenes。使用StringTie软件对mapped reads进行拼接后与参考基因组比对,共发掘6700个新基因。共13869条unigenes在GO数据库中获得注释,分类到3大类45个功能;有10451条unigenes被归纳到KOG数据库25个直系同源功能类型;有13339条unigenes在KEGG得到6大类50小类代谢通路注释。各个组织间差异基因分析显示,肺脏基因表达量数目最多,血液和肌肉差异表达基因数目最多,达5281个,肺脏和血液差异表达基因数目最少,仅249个,分析结果有助于进一步了解雨燕在飞行中的器官功能和代谢调控。研究结果为进一步挖掘雨燕功能基因、分析群体遗传多样性以及探究雨燕能量代谢等方面研究提供参考资料。To enrich the genome data of Beijing swift(Apus apus pekinensis),the Illumina NovaSeq 6000 sequencing platform was used to sequence the transcriptome of muscles,lungs,heart,and blood.The results showed that 32.80 Gb of clean data was obtained after quality control.After Trinity assembly,bioinformatics methods were used to compare with relevant databases,resulting in 15295(71.3%)unigenes with functional annotations.With the StringTie software to con⁃catenate Mapped reads and compare them with the reference genome,a total of 6700 new genes were discovered.A total of 13869 unigenes were annotated in the GO database,classified into three major categories with 45 functions;10451 unigenes were classified into 25 orthologous functional types in the KOG database;13339 unigenes were annotated with six major categories and 50 subcategories of metabolic pathways in KEGG.The differential gene analysis among various tissues showed that the lung had the highest number of gene expression,and the blood and muscle had the highest num⁃ber of differentially expressed genes,reaching 5281.The number of differentially expressed genes in the lung and blood was the least,only 249.The results are helpful to understand the organ function and metabolic regulation of swift during flight.The study provides the reference materials for further exploring functional genes,analyzing population genetic diver⁃sity,and exploring energy metabolism in swifts.

关 键 词：北京雨燕 心脏 肺脏 肌肉 转录组 飞行能量代谢 基因组学 

分 类 号：Q953[生物学—动物学]