肺癌A549细胞来源的IL-17A对破骨细胞分化的作用及分子机制  

The effect and molecular mechanism of IL-17A derived from lung cancer A549 cells on osteoclast differentiation

作　　者：王洪凯 唐浩 袁术杰 梁春涛 WANG Hong-Kai;TANG Hao;YUaN Shu-Jie(Department of Orthopedics,The Second Affiliated Hospital of Guilin Medical University,Guilin 541199,Guangxi,China)

机构地区：[1]桂林医学院第二附属医院骨科,广西桂林541199

出　　处：《中国老年学杂志》2025年第1期109-113,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81960172);广西自然科学基金资助项目(2020GXNSFAA238014);广西医疗卫生重点培育学科建设项目(桂卫科教发[2022]4号)。

摘　　要：目的研究肺癌A549细胞来源的白细胞介素(IL)-17A对破骨细胞分化的作用及分子机制。方法利用慢病毒基因干扰技术建立低表达IL-17A的A549细胞(A549-si)。将A549细胞与破骨细胞前体细胞共培养,观察A549-si对破骨细胞分化的影响;荧光定量聚合酶链反应(qPCR)检测A549-si对破骨细胞分化相关基因和IL-8 mRNA表达的影响;酶联免疫吸附试验(ELISA)检测A549-si对IL-8蛋白表达的影响;将IL-8抗体作用于A549细胞与破骨细胞共培养模型,观察IL-8抗体能否减弱A549细胞对破骨细胞分化的促进作用。结果A549细胞与破骨细胞前体细胞共培养可以明显促进破骨细胞分化,A549-si能够明显抑制A549细胞对破骨细胞分化的促进作用并明显抑制破骨细胞分化相关基因表达(P<0.05);A549-si能够明显抑制A549细胞IL-8 mRNA表达和水平(P<0.05);IL-8抗体能够明显逆转A549细胞对破骨细胞分化的促进作用(P<0.05)。结论A549细胞来源的IL-17A通过促进IL-8表达促进破骨细胞分化。Objective To study the effect of interleukin(IL)-17A derived from lung cancer A549 cells on the differentiation of osteoclast and its molecular mechanism.Methods Lentivirus interference technology was used to construct A549 cells with low expression of IL-17A(A549-si). A549 cells were co-cultured with osteoclast precursor cell to observe the effect of A549-si on osteoclast differentiation. The effect of A549-si on the expression of osteoclast differentiation related genes and IL-8 mRNA was detected by fluorescent quantitative polymerase chain reaction(qPCR). The effect of A549-si on IL-8 protein expression was detected by enzyme linked immunosorbent assay(ELISA). IL-8 antibody was applied to the co-culture model of A549 cells and osteoclast to observe whether IL-8 antibody could weaken the promoting effect of A549 cells on osteoclast differentiation.Results The differentiation of osteoclast was significantly promoted by co-culture of A549 cells with osteoclast precursor cell. The promotion of A549 cells on osteoclast differentiation and the expression of osteoclast differentiation related genes were significantly inhibited by A549-si(P<0.05). The expressions of IL-8 mRNA and protein in A549 cells were significantly inhibited by A549-si;the promoting effect of A549 cell on osteoclast differentiation was significantly reversed by IL-8 antibody(P<0.05). Conclusions IL-17A derived from A549 cells could promote the differentiation of osteoclast by promoting the expression of IL-8.

关 键 词：白细胞介素(IL)-17A 非小细胞肺癌 IL-8 破骨细胞分化 

分 类 号：R73-3[医药卫生—肿瘤]