拉曼光谱成像在牙本质粘接界面研究中的应用  

Application of Raman Spectroscopy Mapping in the Study of Dentin Bonding Interface

作　　者：毕超 刘双双[1] 洪晓黎 许佳佳 李英娘 杨晨 BI Chao;LIU Shuangshuang;HONG Xiaoli;XU Jiajia;LI Yingniang;YANG Chen(Core Facilities,Zhejiang University School of Medicine,Hangzhou 310058,China;Stomatology Hospital,Zhejiang University School of Medicine,Hangzhou 310006,China)

机构地区：[1]浙江大学医学院公共技术平台,杭州310058 [2]浙江大学医学院附属口腔医院,杭州310006

出　　处：《实验室研究与探索》2025年第1期39-43,共5页Research and Exploration In Laboratory

基　　金：浙江省教育厅一般科研项目(Y202250023)。

摘　　要：采用共焦显微拉曼光谱成像技术,以黏接剂-牙本质粘接界面为研究对象,探究非均相体系的化学组成与空间分布。对牙本质与黏接剂分别进行拉曼光谱采集,识别两者的特征峰。对粘接界面某一区域进行拉曼光谱扫描,采用单变量拉曼成像分析法,揭示粘接界面中牙本质矿物质、牙本质胶原成分和黏接剂特征官能团的空间关系与分布;采用多变量拉曼成像分析法,由基于随机森林特征选择的加权模糊C均值聚类算法对高维光谱数据集进行聚类分析,区分粘接界面中牙本质、部分脱矿牙本质、裸露胶原基质、混合层与黏接剂并进行可视化展示。共焦显微拉曼光谱成像技术能够无损、高分辨率地揭示黏接剂-牙本质粘接界面的化学结构和微观形貌,为评估和优化牙本质粘接修复效果提供一种手段。Using confocal micro-Raman spectroscopy mapping,the research focused on the adhesive-dentine interface to explore the chemical compositions and spatial distributions of heterogeneous systems.Raman spectra of dentin and adhesive were collected to identify their respective characteristic peaks.A certain region of the interface was mapped by micro-Raman spectroscopy,and univariate methods of analysis were employed to arrive at the spatial relationships and distributions of the characteristic functional group corresponding to dentin minerals,collagen and adhesive in the interface.Employing multivariate analysis technique,a weighted fuzzy C-means clustering algorithm based on random forest feature selection was used to perform clustering analysis on the hyperspectral data set,clearly distinguishing and visually displaying the various regions of the interface,including dentin,partially demineralized dentin,exposed collagen matrix,hybrid layer,and adhesive.Confocal micro-Raman spectroscopy mapping can nondestructively reveal the chemical structure and microstructure of the adhesive-dentin interface with high resolution,which provides an approach for evaluating and optimizing the effect of dentin adhesive restoration.

关 键 词：牙本质 黏接剂 共焦显微拉曼光谱 拉曼成像 

分 类 号：R783.3[医药卫生—口腔医学] O657.37[医药卫生—临床医学]