理冲生髓饮有效组分通过TRIM44介导NF-κB信号通路对人卵巢癌细胞生物学行为的影响  

Effect of effective components of Lichong Shengsui Yin on biological behavior of human ovarian cancer cells via TRIM44 mediated NF-κB signaling pathway

作　　者：于洋[1,2] 李世颖[3] 韩明轩 郭滢 付杨[2] 韩凤娟 YU Yang;LI Shiying;HAN Mingxuan;GUO Ying;FU Yang;HAN Fengjuan(Postdoctoral Mobile Station of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang,China;The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang,China;Shaoxing Maternal and Child Health Hospital,Shaoxing 312000,Zhejiang,China;Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学博士后流动站,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [3]绍兴市妇幼保健院,浙江绍兴312000 [4]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《现代中西医结合杂志》2025年第1期1-9,17,共10页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(82274566);黑龙江省博士后面上项目(LBH-Z23035);黑龙江省中医药经典普及化专项课题(ZYW2024121)。

摘　　要：目的研究理冲生髓饮有效组分影响人卵巢癌细胞(SKOV3)恶性生物学行为的相关作用机制。方法构建三方基序蛋白44(TRIM44)稳定过表达SKOV3细胞,制备理冲生髓饮有效组分含药血清,CCK-8法检测顺铂的IC50及最佳药物作用浓度;随机将细胞分为空白组、理冲生髓饮有效组分组、顺铂组、联合组,选用细胞划痕法、Transwell法检测细胞迁移、侵袭情况;TUNEL检测细胞凋亡情况;ELISA法及Western blot法检测细胞中相关通路关键因子及蛋白表达情况。结果与空白组比较,不同剂量的理冲生髓饮有效组分作用于TRIM44过表达SKOV3细胞48 h后,细胞活力明显受到抑制,并且抑制率与药物浓度呈正相关(P均<0.05)。与空白组比较,各用药组细胞迁移愈合率均明显降低(P均<0.05),侵袭、迁移细胞数均明显减少(P均<0.05),细胞凋亡数量均明显增加(P均<0.05);联合组细胞迁移愈合率明显低于顺铂组和理冲生髓饮有效组分组(P均<0.05),迁移细胞数、侵袭细胞数均明显少于顺铂组和理冲生髓饮有效组分组(P均<0.05)。各用药组细胞中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-8(IL-8)含量和TRIM44、IκB激酶β(IKKβ)、磷酸化核因子κB抑制蛋白(p-IκBα)、核因子κB p65(NF-κB p65)、B淋巴细胞瘤-2(Bcl-2)、基质金属蛋白酶-9(MMP-9)蛋白相对表达量均明显低于空白组(P均<0.05),核因子κB抑制蛋白(IκBα)、活化半胱氨酸蛋白酶-3(Caspase-3)蛋白相对表达量均明显高于空白组(P均<0.05);各用药组间比较,联合组TNF-α、IL-1β、IL-8含量和TRIM44、IKKβ、NF-κB p65、Bcl-2、MMP-9蛋白相对表达量均明显低于其他2组(P均<0.05),IκBα、Caspase-3蛋白相对表达量均明显高于其他2组(P均<0.05)。结论理冲生髓饮有效组分能够抑制TRIM44过表达SKOV3细胞的增殖、侵袭、转移,促进细胞凋亡,并协同顺铂增强抗肿瘤的作用,其可能通过下调TRIM44表达,抑制NF-κB�Objective It is to investigate the relevant mechanism of the effect of effective components of Lichong Shengsui Yin(LCSSY)on the malignant biological behavior of human ovarian cancer cells(SKOV3).Methods SKOV3 cells with stable overexpression of tripartite motif protein 44(TRIM44)were constructed,and medicated serum containing LCSSY effective component was prepared,the IC50 and optimal drug action concentration of cisplatin were detected by CCK-8 method.The cells were randomly divided into blank group,LCSSY effective component group,cisplatin group,and combination group,the cell migration and invasion were detected by cell scratch and Transwell method,the cell apoptosis was detected by TUNEL method,the expressions of key factors and proteins related to pathways in cells were detected by ELISA and Western Blot method.Results Compared with the blank group,the cell viabilities of SKOV3 cells with TRIM44 overexpression after treated with different doses of LCSSY effective components for 48 hours were significantly inhibited,and the inhibition rate was positively correlated with drug concentration(all P<0.05).Compared with the blank group,the cell migration healing rate of each treatment group was significantly decreased(all P<0.05),the number of invasive and migratory cells were significantly reduced(all P<0.05),and the number of apoptotic cells was significantly increased(all P<0.05),the cell migration healing rate of the combination group was significantly lower than that of the cisplatin group and LCSSY effective component group(all P<0.05),and the number of migratory cells and invasive cells were significantly less than that of the cisplatin group and LCSSY effective component group(all P<0.05).The contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-8(IL-8),and the expressions of TRIM44,I kappa B kinaseβ(IKKβ),phosphorylated nuclear factor kappa B inhibitor protein(p-IκBα),nuclear factor kappa B p65(NF-κB p65),B-lymphomatoma-2(Bcl-2),matrix metalloproteinase-9(MMP-9)in cells o

关 键 词：理冲生髓饮有效组分 人卵巢癌细胞 三方基序蛋白44 NF-ΚB信号通路 恶性生物学行为 

分 类 号：R-33[医药卫生]