RNA-seq转录组测序分析青少年MDD患者外周血lncRNA、miRNA、mRNA表达差异  

Transcriptome sequencing analysis of lncRNA,miRNA and mRNA in peripheral blood from patients with adolescent MDD

作　　者：姜洁 任真奎 刘志国 张珺杰 尹利君 赵强[5] 涂贵兰 孙建超 何军 Jiang Jie;Ren Zhenkui;Liu Zhiguo;Zhang Junjie;Yin Lijun;Zhao Qiang;Tu Guilan;Sun Jianchao;He Jun(Baiyun District People's Hospital of Guiyang,Guiyang 550014,Guizhou,China;The Second People's Hospital of Guizhou Province,Guiyang 550004,Guizhou,China;Guizhou Medical University,Guiyang 561113,Guizhou,China;Zunyi Medical University,Zunyi 563003,Guizhou,China;Guizhou Clinical Laboratory Center,Guiyang 550002,Guizhou,China)

机构地区：[1]贵阳市白云区人民医院,贵州贵阳550014 [2]贵州省第二人民医院,贵州贵阳550004 [3]贵州医科大学,贵州贵阳561113 [4]遵义医科大学,贵州遵义563003 [5]贵州省临床检验中心,贵州贵阳550002

出　　处：《贵州医药》2025年第2期171-176,共6页Guizhou Medical Journal

基　　金：国家自然科学基金(82160311);贵州省卫生健康委科学技术基金(gzwkj2021-256)。

摘　　要：目的RNA-seq转录组测序分析青少年重度抑郁症(major depression disease,MDD)患者外周血差异表达的lncRNA、miRNA、mRNA,为阐明青少年MDD发病机制提供理论补充。方法随机收集样本库4例青少年女性MDD患者与4例正常人对照外周血,TRizol试剂处理后,提取RNA后进行RNA-seq转录组测序,根据P<0.05且|Log2FC|>1计算两组样本的表达差异,绘制差异lncRNA、miRNA、mRNA热图与火山图。通过TargetScan数据库可能预测差异miRNA调控的mRNA,并进行GO功能与KEGG信号通路聚类。结果MDD组和对照组两组间满足P<0.05且|Log2FC|>1差异条件的MDD组中1636个lncRNA上调和825个lncRNA下调,42个miRNAs上调和30个miRNAs下调,2469个mRNA上调和1398个mRNA下调。靶基因主要富集的GO功能:生物学过程(BP)、分子功能(MF)、细胞组分(CC)三个方面。靶基因主要富集的KEGG信号通路:差异表达的lncRNA多富集在Toll-like receptor信号通路、TNF信号通路、Salmonella intection和NF-kappa B信号通路等;差异表达的miRNA多富集在VEGF信号通路、Vasopressin-regulated water reabsorption、Vascular smooth muscle contraction、MAPK信号通路等;差异表达的mRNA靶基因多富集在泛素介导的蛋白质水解、T细胞受体信号通路和MAPK信号通路等。结论本研究结果提供了青少年女性MDD外周血中lncRNA、miRNA、mRNA表达变化的一般情况与可能调控的功能与通路,可能有助于阐明青少年MDD的潜在发病机制。Objective To investigate the altered expression levels of lncRNA,miRNA,and mRNA in peripheral blood mononuclear cells of adolescents diagnosed with major depressive disorder(MDD)by RNA-seq transcriptome sequencing,provide a theoretical supplement to elucidate the pathogenesis of MDD in adolescents.Methods It was randomly selected four adolescent female MDD patients alongside four healthy controls,collecting their PBMCs for analysis and extracted RNA using TRIzol reagent.This was followed by RNA-seq transcriptome sequencing.There was statistically significant expression differences between the two groups were assessed based on a significance threshold of P<0.05 and|log2FC|>1.Differentially expressed lncRNAs,miRNAs,and mRNAs were visualized through heatmaps and volcano plots.Target genes for differentially expressed miRNAs were predicted utilizing the TargetScan database.Subsequently,Gene Ontology(GO)functional enrichment and KEGG pathway analyses were conducted.Results In the MDD cohort,1636 lncrnas were up-regulated and 825 lncRNAs were down-regulated,42 miRNAs were up-regulated and 30 miRNAs were down-regulated,2469 mRNA were up-regulated and 1398 mRNA were down-regulated.The target genes were classified into three main categories of Gene Ontology(GO)functions:biological process(BP),molecular function(MF),and cellular component(CC).Furthermore,these target genes demonstrated significant enrichment in various KEGG signaling pathways that differentially expressed lncRNAs primarily associated with the Toll-like receptor signaling pathway,TNF signaling pathway,Salmonella infection pathways,and NF-kappa B signaling pathway.Differentially expressed miRNAs mainly enriched in the VEGF signaling pathway as well as vasopressin-regulated water reabsorption pathways.While differentially expressed mRNA target genes significantly clustered around ubiquitin-mediated protein degradation processes along with T cell receptor signaling pathways and MAPK signaling pathways.Conclusion This study provides an overview of the regulator

关 键 词：青少年MDD RNA-seq转录组测序 LncRNA micoRNA mRNA 

分 类 号：R749.4[医药卫生—神经病学与精神病学]