GB 5009.89高效液相色谱法检测含乳酸菌奶粉中维生素B3的问题研究  

Transformation issue in using GB 5009.86 high performance liquid chromatography method to determine vitamin B3 for milk powder containing Lactic acid bacteria

作　　者：姜勇 高元娇 张秀宇 张凤艳 靳琳琳 王曼霞 何飞 雷质文 JIANG Yong;GAO Yuan-Jiao;ZHANG Xiu-Yu;ZHANG Feng-Yan;JIN Lin-Lin;WANG Man-Xia;HE Fei;LEI Zhi-Wen(Danone Specialized Nutrition(Qingdao)Co.,Ltd.,Qingdao 266113,China;Qingdao Customs Technology Center,Qingdao 266109,China;Beijing Xinrui Haoyang Technology Co.,Ltd.,Beijing 100070,China;Qingdao Food and Drug Inspection Institute,Qingdao 266071,China)

机构地区：[1]达能特殊营养品(青岛)有限公司,青岛266113 [2]青岛海关技术中心,青岛266109 [3]北京信睿浩扬科技有限公司,北京100070 [4]青岛市食品药品检验研究院,青岛266071

出　　处：《实验室检测》2024年第8期140-145,共6页Laboratory Testing

摘　　要：目的 研究使用GB 5009.89-2016第二法高效液相色谱法检测含乳酸菌的奶粉中维生素B3含量时,发生的烟酰胺转化为烟酸的现象,探讨其机理。方法 样品按照标准方法程序进行前处理,通过单因素实验分别考察了试样测定液静置时间、溶液酸水解前静置时间以及酸水解pH值对结果的影响。结果 烟酰胺向烟酸的转化发生在样品溶解后、酸水解之前的静置步骤,但调节pH值至标准规定的1.7进行酸水解以后,转化作用被完全抑制。结论 如果要检测含乳酸菌样品中烟酸或烟酰胺的含量,需要在样品溶解时立即将pH值调整到1.7,以抑制烟酰胺向烟酸的转化。Objective Study why nicotinamide is transformed to nicotinic acid occurred when detect vitamin B3 in milk powder containing lactic acid bacteria by GB 5009.89-2016 high performance liquid chromatography method,and its mechanism was discussed.Methods The samples were pretreated according to standard procedures,and the effects of the placement time of the sample extraction solution,the placement time of the sample solution before acid hydrolysis and the pH value of acid hydrolysis were investigated by single factor test.Results The transformation of nicotinamide to nicotinic acid occurred in the placement step after the sample was dissolved and before acid hydrolysis,but after adjusting the pH value to 1.7 specified in the standard for acid hydrolysis,the transformation was completely inhibited.Conclusion If you want to detect the content of nicotinic acid or nicotinamide separately in samples containing lactic acid bacteria,you need to adjust the pH value to 1.7 when the sample is dissolved to inhibit the transformation of nicotinamide to nicotinic acid.

关 键 词：烟酸 烟酰胺 GB 5009.89 乳酸菌 

分 类 号：O657.72[理学—分析化学] TS252.51[理学—化学]